We narrowed to 1,516 results for: SIR

1. pFRT-TO-RPB1-His CRres K619,627,642R si2,4R

   Plasmid

   #139409

   Purpose

   Dox-inducible, K619, 627, 642R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

   Depositor

   Jesper Svejstrup

   Article

   Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

   Insert

   RPB1 (POLR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   K619, 627, 642R

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFRT-TO-RPB1-His K751,758,767R CRres si2,4R

   Plasmid

   #139408

   Purpose

   Dox-inducible, K751, 758, 767R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

   Depositor

   Jesper Svejstrup

   Article

   Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

   Insert

   RPB1 (POLR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   K751, 758, 767R

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFRT-TO-RPB1-His K710,719R CRres si2,4R

   Plasmid

   #139407

   Purpose

   Dox-inducible, K710, 719R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

   Depositor

   Jesper Svejstrup

   Article

   Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

   Insert

   RPB1 (POLR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   K710,719R

   Promoter

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBrain-GFP-TACC3KDP∆678-681-shTACC3

   Plasmid

   #69115

   Purpose

   Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Inserts

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   shTACC3
   

   Use

   RNAi

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of 678-681aa region. Silent mutations i…

   Promoter

   CMV

   Available since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-mCherry-KATNA1, V55D

   Plasmid

   #180651

   Purpose

   Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Has V55D mutation. Dox-inducible. Internal ID: WISP20-48.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRNA resistant to: GGACAGCACUCCCUUGAAA

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX-6P-1-Eps8L2_1-496

   Plasmid

   #196516

   Purpose

   Expression of GST-Eps8L2_1-496

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2_1-496 (EPS8L2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Silent mutations for siRNA resistance

   Promoter

   lac promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pD649-HAsp-VSTM1-COMP5AP-AviTag-9xHis

   Plasmid

   #157060

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   VSTM1 (VSTM1 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiCMV-Blast mLST8-Flag Mut1-2

   Plasmid

   #124918

   Purpose

   For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L and W272F/W274F mutant

   Depositor

   Jin Chen

   Article

   Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

   Insert

   MLST8 (MLST8 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Insert ORF was sirently mutated to be resistent t…

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCherry-POLE1

   Plasmid

   #215140

   Purpose

   Expresses mCherry-POLE1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   POLE1 (POLE Human)
   

   Use

   Retroviral

   Tags

   mCherry_FWR

   Expression

   Mammalian

   Mutation

   Silent mutations in amino acid 80-86 and 211-217 …

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FUW-tetO-wtYAP

    Plasmid

    #84009

    Purpose

    expresses wtYAP in mammalian cells under the control of a doxycycline-inducible promoter

    Depositor

    Stefano Piccolo

    Article

    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

    Insert

    YAP1 (siRNA insensitive) (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-CMV

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge