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pTETDuet_M42FG121VDHFR_R166QTS

Plasmid

#191298

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

M42F/G121V DHFR, R166Q TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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EcYbbN pET15b

Plasmid

#60690

Purpose

Recombinant protein expression of YbbN

Depositor

Mark Wilson

Article

Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

Insert

YbbN

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pMAM-RecG-ST2TaV-EGFP

Plasmid

#31275

Depositor

Andrew Pierce

Article

Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

Insert

RecG-ST2-TaV-EGFP

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMAM-RecG-EGFP

Plasmid

#31274

Depositor

Andrew Pierce

Article

Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

Insert

RecG-EGFP

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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MetNI (Lm)

Plasmid

#78316

Purpose

Methionine ABC Transporter

Depositor

Douglas Rees

Article

Kadaba et al Science. 2008 Jul 11;321(5886):250-3. do

Inserts

MetI
MetN

Tags

10x Histidine Tag and 6x Histidine Tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD33(A-N)

Plasmid

#187084

Purpose

Medium expression of Complex I with subunit N-HA tagged

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoA-N

Tags

HA tag nuoN

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD33(EFG)

Plasmid

#187087

Purpose

Medium expression of subunits EFG

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoEFG

Tags

HA tag nuoG

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAW209

Plasmid

#113188

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pTETDuet_WTDHFR_WTTS

Plasmid

#191282

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

WT DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSPK1

Plasmid

#18876

Depositor

Jay Keasling

Article

Van Dien et al Appl Environ Microbiol. 1997 May . 63(5)

Insert

polyphosphate kinase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only
PAS513 (TJF080)

Plasmid

#65580

Purpose

pETDuet-1 Containing FadD S379R

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Expression

Bacterial

Mutation

S379R

Promoter

T7 lacO

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
PAS529 (TJF072)

Plasmid

#65603

Purpose

pETDuet-1 Containing FadD Q338R-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Tags

6x His

Expression

Bacterial

Mutation

Q338R

Promoter

T7 lacO

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
PAS504 (TJF067)

Plasmid

#65571

Purpose

pETDuet-1 Containing FadD F447S

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD with F447S mutation

Expression

Bacterial

Mutation

F447S

Promoter

T7 lacO

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
pUPD3_CDS_lacI

Plasmid

#244017

Purpose

GoldenBraid2.0 IPTG/Lactose-dependent transcriptional repressor.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

lacI

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_CDS_tetR

Plasmid

#244019

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-dependent transcriptional repressor.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_lacI_Plac

Plasmid

#243920

Purpose

GoldenBraid2.0 IPTG/Lactose-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

lacI and PlacZYA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_tetR_Ptet

Plasmid

#243924

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetR and PtetA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_cI857_PR

Plasmid

#243926

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cI857 and PR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_PR

Plasmid

#243927

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, not including ribosome binding site. Lacks repressor, cI857.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PR without cI857

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_PT7

Plasmid

#243928

Purpose

GoldenBraid2.0 T7 RNA polymerase-dependent promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_PT7lac

Plasmid

#243929

Purpose

GoldenBraid2.0 T7 RNA polymerase and IPTG/Lactose-dependent promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7lac

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_lacI_Plac

Plasmid

#243909

Purpose

GoldenBraid2.0 IPTG/Lactose-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

lacI and PlacZYA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_araC_Para

Plasmid

#243911

Purpose

GoldenBraid2.0 Arabinose-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

araC and ParaBAD

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_tetR_Ptet

Plasmid

#243913

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetR and PtetA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_cI857_PR

Plasmid

#243915

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cI857 and PR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_PR

Plasmid

#243916

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, including ribosome binding site. Lacks repressor, cI857.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PR without cI857

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD3_FULLPRM_PT7

Plasmid

#243917

Purpose

GoldenBraid2.0 T7 RNA polymerase-dependent promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD3_FULLPRM_PT7lac

Plasmid

#243918

Purpose

GoldenBraid2.0 T7 RNA polymerase and IPTG/Lactose-dependent promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7lac

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pHK346

Plasmid

#235477

Purpose

Inducible gene VIII by Pbad promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII

Expression

Bacterial

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHK336

Plasmid

#235476

Purpose

Inducible gene VIII by Plux promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII

Expression

Bacterial

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGU06

Plasmid

#212148

Purpose

Plasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.

Depositor

Kang Zhou

Insert

alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase, tyrosine aminotransferase (AGX1, alaA, tyrB Budding Yeast, Agx1: S. cerevisiae; alaA, tyrB: E. coli)

Expression

Bacterial

Promoter

PgyrA

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_F31YDHFR_R127ATS

Plasmid

#191312

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

F31Y DHFR/R127A TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_D27NDHFR_Q33STS

Plasmid

#191289

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

D27N DHFR, Q33S TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_M42FG121VDHFR_Q33STS

Plasmid

#191292

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

M42F/G121V DHFR, Q33S TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_F31YDHFR_R166QTS

Plasmid

#191311

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

F31Y DHFR/R166Q TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_F31YDHFR_WTTS

Plasmid

#191310

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

F31Y DHFR/WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_F31YDHFR_Q33STS

Plasmid

#191313

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

F31Y DHFR/Q33S TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_F31YG121VDHFR_Q33STS

Plasmid

#191293

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

F31Y/G121V DHFR, Q33S TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_WTDHFR_R166QTS

Plasmid

#191294

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

WT DHFR, R166Q TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_D27NDHFR_R166QTS

Plasmid

#191295

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

D27N DHFR, R166Q TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 561 - 600 of 10322 results

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