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pEMBL-Si-EGFP

Plasmid

#20692

Depositor

Tom Misteli

Article

Mejat et al J Cell Biol. 2009 Jan 12. 184(1):31-44.

Insert

U6-siEGFP

Use

Adeno associated virus

Expression

Mammalian

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pEMB36(revcomp)/Dvl1_1_670

Plasmid

#40557

Depositor

Michael J Fox Foundation MJFF

Insert

DVL1 (DVL1 Human)

Use

Baculovirus

Mutation

WT; aa1_670

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)

Plasmid

#92400

Purpose

Constitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and membrane tag

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)

Plasmid

#92401

Purpose

Constitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and membrane tag

Promoter

bactin2

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEMB52 / Anemone_LRRK2_1_334

Plasmid

#40550

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2

Use

Baculovirus

Tags

6xHis

Mutation

WT

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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Nano-lantern-plasma membrane/pcDNA3

Plasmid

#51975

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Tags

HIS and Lyn

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
membrane-HsPLK1

Plasmid

#185571

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1 (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEMB12 LRRK2_BC_1847_2210_K1906M

Plasmid

#40564

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

aa1847_2210; K1906M

Promoter

polH

Available Since

Oct. 11, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 / LRRK2_BC_1333_2187

Plasmid

#40510

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

WT; aa1333_2187

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 / LRRK2_BC_1500_2170

Plasmid

#40514

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

WT; aa1500_2170

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 / LRRK2_BC_1309_2527_M2397T

Plasmid

#40523

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

aa1309_2527; M2397T

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 / LRRK2_BC_1309_2350

Plasmid

#40529

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

WT; aa1309_2350

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 / LRRK2_BC_1500_2232

Plasmid

#40531

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

WT; aa1500_2232

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 / LRRK2_BC_1882_2350

Plasmid

#40535

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

WT; aa1882_2350

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 / LRRK2_BC_1185_2136

Plasmid

#40539

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

WT; aa1185_2136

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB52/Armadillo_34_373

Plasmid

#40543

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2

Use

Baculovirus

Tags

6xHis

Mutation

WT

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB52/Horse_34_374

Plasmid

#40544

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2

Use

Baculovirus

Tags

6xHis

Mutation

WT

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB52/Turkey_34_374

Plasmid

#40545

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2

Use

Baculovirus

Tags

6xHis

Mutation

WT

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB52 / Urchin_LRRK2_1_335

Plasmid

#40551

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2

Use

Baculovirus

Tags

6xHis

Mutation

WT

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB44 / Lancelet_LRRK2_1_319

Plasmid

#40554

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2

Use

Baculovirus

Tags

6xHis

Mutation

WT

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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