We narrowed to 1,041 results for: pEGFP-N

1. Venus-Bik-G154STOP-pEGFP-C1

   Plasmid

   #177428

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.

   Depositor

   David Andrews

   Insert

   Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Glycine 154 replaced with STOP codon

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP-N1 hMOG-beta2-EGFP

   Plasmid

   #160979

   Purpose

   Expresses a human myelin oligodendrocyte glycoprotein isoform beta2 - EGFP fusion protein in mammalian cells

   Depositor

   Markus Reindl

   Article

   Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

   Insert

   Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta2 (MOG Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-C1-G3BP1-DeltaRBP

   Plasmid

   #135999

   Purpose

   G3BP1 with RNA binding domains deleted inserted with GFP tagged on the N-terminus

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C-term of G3BP1 Deleted for Delta RBP

   Promoter

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-C1-G3BP1-S149A

   Plasmid

   #135998

   Purpose

   S149A G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S149A

   Promoter

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP-Flag-stm2585

   Plasmid

   #174397

   Purpose

   Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags.

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   sarA
   

   Use

   Tags

   EGFP and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Venus-BclXL-pEGFP-C1

   Plasmid

   #177409

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

   Depositor

   David Andrews

   Article

   Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

   Insert

   Bcl-XL
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-Rab21-T31N (DN)

   Plasmid

   #83423

   Purpose

   Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.

   Depositor

   Johanna Ivaska

   Article

   Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

   Insert

   Rab21 (Rab21 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Residue 31 from T to N (T31N: ACG -> AAC)

   Promoter

   CMV

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCerulean3-BclXL-pEGFP-C1

   Plasmid

   #177408

   Purpose

   To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

   Depositor

   David Andrews

   Article

   Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

   Insert

   Bcl-XL
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-CD86-mApple

   Plasmid

   #133860

   Purpose

   Mammalian expression of CD86 N-terminally tagged with EGFP and C-terminally tagged with mApple

   Depositor

   Dave Piston

   Article

   Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

   Insert

   CD86 (CD86 Human)
   

   Use

   Tags

   EGFP and mApple

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-Flag-stm2585_S130A

    Plasmid

    #174399

    Purpose

    Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its GSK3-binding SxxxSP motif mutated to SxxxAP

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA
    

    Use

    Tags

    EGFP and Flag

    Expression

    Mammalian

    Mutation

    S130A

    Promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-Flag-stm2585_Y167F

    Plasmid

    #174398

    Purpose

    Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQ

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA
    

    Use

    Tags

    EGFP and Flag

    Expression

    Mammalian

    Mutation

    Y167F

    Promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-C1-hFGD5

    Plasmid

    #50760

    Purpose

    expression of EGFP-tagged human FGD5

    Depositor

    Naoki Mochizuki

    Article

    Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

    Insert

    FGD5 (FGD5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Venus-Bik-cb5-pEGFP-C1

    Plasmid

    #177431

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Bik MBR removed (after position 136) and replaced…

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-Sec23A

    Plasmid

    #66609

    Purpose

    GFP fusion of Sec23A

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec23A (SEC23A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-RNF17L

    Plasmid

    #35413

    Depositor

    Jeremy Wang

    Article

    Pan et al Development. 2005 Sep;132(18):4029-39. E

    Insert

    Rnf17l (Rnf17 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pEGFP-RNF17S

    Plasmid

    #35414

    Depositor

    Jeremy Wang

    Article

    Pan et al Development. 2005 Sep;132(18):4029-39. E

    Insert

    Rnf17s (Rnf17 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pEGFP-C1-hAtg2A

    Plasmid

    #36456

    Depositor

    Noboru Mizushima

    Article

    Velikkakath et al Mol Biol Cell. 2012 Mar;23(5):896-909. E

    Insert

    autophagy-related protein 2 homolog A (ATG2A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed Proline 656 to Arginine

    Promoter

    CMV

    Available Since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N1-Cdc42BPB

    Plasmid

    #50759

    Purpose

    expression of EGFP tagged Cdc42BPB

    Depositor

    Naoki Mochizuki

    Article

    Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

    Insert

    Cdc42BPB (CDC42BPB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TMEM16F-I521K-peGFP-N1

    Plasmid

    #228588

    Purpose

    Expresses TMEM16F-I521K-peGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    I521K plus a 3 amino acid N-terminal truncation (…

    Promoter

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TMEM16F-I611K-peGFP-N1

    Plasmid

    #228581

    Purpose

    Expresses mouse TMEM16F-I611K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I611K plus a 3 amino acid N-terminal truncation (…

    Promoter

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge