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Plasmid#185780PurposeEncodes enzyme bovine carbonic anhydrase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-P(11)4
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-API5
Plasmid#157655PurposeExpresses API5 in E.coli cellDepositorInsertApoptosis inhibitor 5 (API5 Human)
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-T5-ASG_ClpXP
Plasmid#153299PurposeExpresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917DepositorInsertASG
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MseLig1
Plasmid#107071PurposeExpresses M. sedula DNA Ligase 1 in E. coliDepositorInsertDNA ligase 1
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-AF0623
Plasmid#30164DepositorInsertAF0623 (lig Archaeoglobus fulgidus)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-P5CR
Plasmid#110295PurposeExpresses pyrroline-5-carboxylate reductase in E. coli BL21(DE3)DepositorInsertPyrroline-5-carboxylate Reductase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-EgC*
Plasmid#20378DepositorInsertEglin C wt*
UseTagsExpressionBacterialMutationF10WPromoterAvailable sinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28-GG-PUF
Plasmid#52137PurposeReceiving vector for PUM-HD assembly and expression in E.coliDepositorTypeEmpty backboneUseTagsPUM-HD flanking region 1' and PUM-HD flankin…ExpressionBacterialMutationPromoterT7Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28b-Rv2416c_wild
Plasmid#37649DepositorInsertRv2416c (eis Mycobacterium tuberculosis)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-λN-βLac
Plasmid#162039PurposeExpresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)DepositorInsertλN domain of the λ phage and a β-lactamase fusion protein
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbB
Plasmid#114155PurposeExpresses full-length ClbBDepositorInsertclbB
UseTags6xHis-thrombin-T7 tagExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mxLOX1
Plasmid#104975PurposeBiosynthesis of oxylipins by microbial enzymesDepositorInsertMXAN_1745 (MXAN_1745 Myxococcus xanthus)
UseTags6 histidine tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mxLOX2
Plasmid#104976PurposeBiosynthesis of oxylipins by microbial enzymesDepositorInsertMXAN_1744 (MXAN_1744 Myxococcus xanthus)
UseTags6 histidine tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-pchA WT
Plasmid#87102Purposeexpresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosaDepositorInsertpchA WT
UseTagshis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (P to A)
Plasmid#62069PurposeExpression of human p53 (1-73) (all P to A) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains TAD residues 1-73; all Prolines changed …PromoterT7Available sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28GSTlinker-LIC
Plasmid#119970PurposeStructure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28(a)-hFX-HA
Plasmid#14991DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1, HA, and HISExpressionBacterialMutationcontains cDNA coding sequence for amino acids 88-…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)/FbGH30
Plasmid#86463PurposeExpression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coliDepositorInsertFbGH30
UseTagsHis-TagExpressionBacterialMutationPromoterT7Available sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)/FaGH17A
Plasmid#86462PurposeExpression of the Beta-1,3-Endo-Laminarinase FaGH17A in E.coliDepositorInsertFaGH17A
UseTagsHis-TagExpressionBacterialMutationPromoterT7Available sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-Ava3856
Plasmid#45742DepositorInsertAva3856 (Ava_3856 Anabaena variabilis)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only