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Showing: 281 - 300 of 521 results

  1. GGE094

    Plasmid
    #120787
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsDown_Zeta-SfiI
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120787-plasmid-map-sequence-id-263185

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  2. GGE091

    Plasmid
    #120786
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsUp_Zeta-SfiI
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120786-plasmid-map-sequence-id-263184

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  3. GGE254

    Plasmid
    #120789
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsDown_Lip-NotI
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    120789-plasmid-map-sequence-id-263222

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  4. GGE255

    Plasmid
    #120788
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsUp_Lip-NotI
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    120788-plasmid-map-sequence-id-310818

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  5. GGE207

    Plasmid
    #120792
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsDown-Mfe
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    120792-plasmid-map-sequence-id-263227

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  6. KPC2-pNic-Bsa4

    Plasmid
    #231082
    Purpose
    expresses Klebsiella pneumoniae carbapenemase-2 (KPC-2) under T7 with His tag
    Depositor
    Josh Beckham
    Insert
    carbapenemase-2 (kpc2 )
    Use
    Tags
    6x His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    231082-plasmid-map-sequence-id-462636

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  7. TC9nickpAB

    Plasmid
    #71311
    Purpose
    used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI
    Depositor
    Karl Degenhardt
    Insert
    Cas9 nickase
    Use
    In vitro transcription
    Tags
    NLS
    Expression
    Mutation
    D10A nickase
    Promoter
    T7
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    71311-plasmid-map-sequence-id-131863

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  15. GGE020

    Plasmid
    #120777
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position1-3. When using the GG platform for expressing only one gene (T1-3_Lip2)
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    T1-3_Lip2
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120777-plasmid-map-sequence-id-263162

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  16. GGE021

    Plasmid
    #120778
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position2-3. When using the GG platform for expressing only two gene (T2-3_Lip2)
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    T2-3_Lip2
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    120778-plasmid-map-sequence-id-263164

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  17. pTOPO-col10a1-KI-donor

    Plasmid
    #184874
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Christoph Winkler
    Article
    Hui et al Development. 2022 May 20. pii: 275483. d
    Inserts
    Collagen10a1 5' homology arm
    Collagen10a1 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    eGFP
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    184874-plasmid-map-sequence-id-363155

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  19. pGL4_CRE-CMVmin-luc2

    Plasmid
    #194384
    Purpose
    Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter
    Depositor
    Michael Wehr
    Article
    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48
    Insert
    6xCRE-CMVmin-luc2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV minimal
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194384-plasmid-map-sequence-id-381615

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  20. Lmx1a-in situ probe

    Plasmid
    #45071
    Depositor
    Kathleen Millen
    Insert
    Lmx1a LIM homeobox transcription factor 1 alpha (residues 94-1149) (Lmx1a Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Lmx1a residues 94-1149
    Promoter
    Available since
    May 30, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 281 - 300 of 521 results