We narrowed to 19,837 results for: ada

1. CamKII-LGI1-E383A-pHluorin

   Plasmid

   #185544

   Purpose

   Expresses mutant E383A LGI1-pHluorin under the CamKII promoter

   Depositor

   Jaime de Juan-Sanz

   Article

   Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

   Insert

   LGI1 (Lgi1 Rat)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   changed glutamic acid 383 for alanine

   Promoter

   CamKII

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CamKII-LGI1-C200R-pHluorin

   Plasmid

   #185545

   Purpose

   Expresses mutant C200R LGI1-pHluorin under the CamKII promoter

   Depositor

   Jaime de Juan-Sanz

   Article

   Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

   Insert

   LGI1 (Lgi1 Rat)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   changed cysteine 383 for arginine

   Promoter

   CamKII

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-PE7-P2A-hMLH1dn

   Plasmid

   #222997

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-hMLH1dn
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Deletion of residues 754-756 in MLH1 as described…

   Promoter

   CMV

   Available since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-PE7-P2A-hMLH1dn

   Plasmid

   #222997

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-hMLH1dn
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Deletion of residues 754-756 in MLH1 as described…

   Promoter

   CMV

   Available since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKT2/CAGXSP/CD63mScarlet

   Plasmid

   #182972

   Purpose

   Stably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.

   Depositor

   Masamitsu Kanada

   Article

   Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

   Insert

   CD63-mScarlet (CD63 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKT2/CAGXSP/CD63mScarlet

   Plasmid

   #182972

   Purpose

   Stably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.

   Depositor

   Masamitsu Kanada

   Article

   Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

   Insert

   CD63-mScarlet (CD63 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EK0438 SFFV-mCherry-SGc(s70587)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191163

   Purpose

   Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:s70587:EGFP-3xMS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EK0438 SFFV-mCherry-SGc(s70587)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191163

   Purpose

   Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:s70587:EGFP-3xMS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGGG-AH-VRT-A2

   Plasmid

   #163703

   Purpose

   Wheat transformation vector pGoldenGreenGate (pGGG) with OsActinP:: hygromycin (hpt) selection and the full native Triticum polonicum VRT-A2 gene (native prom::genomic seq::3'UTR)

   Depositor

   Cristobal Uauy

   Article

   Adamski et al Plant Cell. 2021 Aug 13;33(7):2296-2319.

   Inserts

   Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
   Triticum polonicum VRT-A2 genomic sequence (Native promoter:: genomic sequence::3'UTR) + Nos Terminator
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Rice - Os Actin promoter and Triticum polonicum V…

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGGG-AH-VRT-A2

    Plasmid

    #163703

    Purpose

    Wheat transformation vector pGoldenGreenGate (pGGG) with OsActinP:: hygromycin (hpt) selection and the full native Triticum polonicum VRT-A2 gene (native prom::genomic seq::3'UTR)

    Depositor

    Cristobal Uauy

    Article

    Adamski et al Plant Cell. 2021 Aug 13;33(7):2296-2319.

    Inserts

    Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
    Triticum polonicum VRT-A2 genomic sequence (Native promoter:: genomic sequence::3'UTR) + Nos Terminator
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Rice - Os Actin promoter and Triticum polonicum V…

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTRIP-MND-HsMAN1C1-1-630-cMycFlag-hPGK-GFP

    Plasmid

    #183505

    Purpose

    Express GFP and MAN1C1 proteins

    Depositor

    Olivier Guipaud

    Article

    Ladaigue et al iScience. 2022 Nov 2;25(12):105482. doi:

    Inserts

    man1C1 (MAN1C1 Human)
    EGFP
    

    Use

    Lentiviral

    Tags

    Myc-DDK

    Expression

    Mammalian

    Mutation

    Promoter

    MND and hPGK

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTRIP-MND-HsMAN1C1-1-630-cMycFlag-hPGK-GFP

    Plasmid

    #183505

    Purpose

    Express GFP and MAN1C1 proteins

    Depositor

    Olivier Guipaud

    Article

    Ladaigue et al iScience. 2022 Nov 2;25(12):105482. doi:

    Inserts

    man1C1 (MAN1C1 Human)
    EGFP
    

    Use

    Lentiviral

    Tags

    Myc-DDK

    Expression

    Mammalian

    Mutation

    Promoter

    MND and hPGK

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. G15-CASE

    Plasmid

    #168126

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at E244/N245 within GNA15

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. G15-CASE

    Plasmid

    #168126

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at E244/N245 within GNA15

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-Lamin-A

    Plasmid

    #206027

    Purpose

    Expresses GFP-tagged Lamin A in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Lamin-A (LMNA Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-Lamin-A

    Plasmid

    #206027

    Purpose

    Expresses GFP-tagged Lamin A in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Lamin-A (LMNA Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Gi3-CASE

    Plasmid

    #168122

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at A114/E115 within GNAI1

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Gi3-CASE

    Plasmid

    #168122

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at A114/E115 within GNAI1

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Gq-CASE

    Plasmid

    #168125

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at F124/E125 within GNAQ

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Gq-CASE

    Plasmid

    #168125

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at F124/E125 within GNAQ

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge