We narrowed to 6,270 results for: arl

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pAAVS1-TetOn-dCas9-KRAB

Plasmid

#115545

Purpose

Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

Depositor

Rene Maehr

Article

Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

Type

Empty backbone

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPathBrick

Plasmid

#65006

Purpose

E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

Depositor

Mattheos Koffas

Article

Cress et al ACS Synth Biol. 2015 Mar 30.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Constitutive native promoters

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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ABCG1_pCSdest

Plasmid

#53952

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ABCG1 (ABCG1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pCDNA3.1-hMcl-1

Plasmid

#25375

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

MCL1 (MCL1 Human)

Tags

V5 and His tag

Expression

Mammalian

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pCM189-ParB1::mCherry

Plasmid

#87253

Purpose

Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

ParB1

Tags

mCherry

Expression

Yeast

Promoter

pBAD/tetO

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-3m160-HilA

Plasmid

#153325

Purpose

Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3m160 DARPin

Use

Salmonella expression

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_empty

Plasmid

#203811

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pJR022

Plasmid

#198820

Purpose

C. elegans optimized version of mScarlet

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::wrmScarlet::let-858 3'UTR

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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DsRed-Rab5 WT

Plasmid

#13050

Depositor

Richard Pagano

Article

Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

Insert

RAB5A (Canis familiaris) (RAB5A Canis familiaris)

Tags

DsRed2 and HA

Expression

Mammalian

Mutation

asparagine 3 changed to serine (neutral); serine …

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRPV1exCellHalo

Plasmid

#220307

Purpose

Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

Depositor

Eric Senning

Article

Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

Insert

rTRPV1exCellHalo (Trpv1 Rat)

Tags

circularly permutated Halotag (Deo et al., Nat Ch…

Expression

Mammalian

Mutation

linker included to insert cpHaloTag (

Promoter

CMV promoter

Available Since

July 30, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

Plasmid

#84246

Purpose

Expresses direct fusion VPR-Sa dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sa dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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Profiline

Plasmid

#89925

Purpose

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin

Depositor

Antoine Jégou, Guillaume Romet-Lemonne

Article

Jegou et al PLoS Biol. 2011 Sep;9(9):e1001161. doi:

Insert

profiline-1 (PFN1 Human)

Tags

None

Expression

Bacterial

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-eSpCas9(1.1)

Plasmid

#108292

Purpose

pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_MYRF

Plasmid

#64843

Purpose

A lentiviral vector for the expression of mouse MYRF under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

MYRF (Myrf Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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sg2.02 16x(MS2) MUC4.1

Plasmid

#101154

Purpose

gRNA with 16 MCP binding site

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL2

Plasmid

#109020

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2 (ATL2 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

EIF-1a promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Olig2

Plasmid

#64842

Purpose

A lentiviral vector for the expression of mouse Olig2 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Olig2 (Olig2 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCASB

Plasmid

#190175

Purpose

Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

Depositor

Jessica Tyler

Article

Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

Insert

sgRNA cloning site

Expression

Bacterial and Yeast

Mutation

Added 5'-agagacc-3' upstream and 5'…

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

Plasmid

#84245

Purpose

Expresses direct fusion KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

KRAB-tagBFP-Sp dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), HA tag, KRAB, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-DEST

Plasmid

#106348

Purpose

Destination vector for gateway cloning. Developed for Flp-In T-REx system.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Type

Empty backbone

Use

Gateway entry vector

Expression

Mammalian

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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