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Tol2-LyzC-TagRFP-T-EEA1

Plasmid

#254776

Purpose

Neutrophil specific Early Endosome Antigen 1 expression in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

EEA1 (EEA1 Human)

Use

Zebrafish expression

Tags

TagRFP-T

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pET29b(+)_SUMO(1-98)_sTEV_His6

Plasmid

#254139

Purpose

Plasmid for expressing Control protein construct of the AH-SUMO platfrom, without any N-terminal inducible amphipathic helix.

Depositor

Peter Chung

Article

Margaritakis et al Commun Chem. 2026 Apr 2;9(1):165. doi: 1

Insert

SUMO family protein SMT3 (SMT3 Budding Yeast)

Expression

Bacterial

Mutation

Contains MGSGS sequence on N-terminal of SMT3_yea…

Available Since

April 28, 2026

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIC3_cpGR2(GGS5)-F59

Plasmid

#246053

Purpose

Expression of AcrIIC3-cpGR2 hybrid in mammalian cells; insertion before F59; enables chemogenetic control of NmeCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIC3_cpGR2-F59

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_cpGR2(GGS5)-N77

Plasmid

#246046

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before N77; enables chemogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-N77

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_cpGR2(GGS5)-D41

Plasmid

#246045

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before D41; enables chemogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-D41

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_AsLOV2-N77

Plasmid

#246044

Purpose

Expression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before N77; enables optogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_AsLOV2-N77

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_AsLOV2-D41

Plasmid

#246043

Purpose

Expression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before D41; enables optogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_AsLOV2-D41

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1837

Plasmid

#239840

Purpose

7. BK1837- pLV-CMV-SNCAintron1-AlphaSyn-d2EGFP-P2A-NLuc-SV40-Puro-WPRE

Depositor

Boris Kantor

Article

Kantor et al Mol Ther. 2018 Nov 7;26(11):2638-2649. d

Inserts

pLV-CMV-SNCAintron1-AlphaSyn-d2EGFP-P2A-NLuc-SV40-Puro-WPRE (pCL35)
pLV-CMV-SNCAintron1-AlphaSyn-d2EGFP-P2A-NLuc-SV40-Puro-WPRE (pCL35)

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pREC1010-Eco2

Plasmid

#240681

Purpose

RSF1010 origin of replication plasmid containing Eco2 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Eco2 RT, Eco2 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Eco1

Plasmid

#240705

Purpose

pLAM12 vector backbone containing Eco1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Eco1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pNF-KB-Split PS Intein-C tTA

Plasmid

#242035

Purpose

C-terminal segment of a blue-light-controlled split PS Intein tTA gene expression system, co-expressing mCherry under an NF-KB-inducible promoter

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Split PS Intein-C tTA

Tags

mCherry

Expression

Mammalian

Mutation

The CMV promoter in the original vector was repla…

Promoter

NF-KB

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Eco2

Plasmid

#240706

Purpose

pLAM12 vector backbone containing Eco2 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Eco2 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pREC1010lac-Eco1

Plasmid

#240691

Purpose

RSF1010 origin of replication plasmid containing Eco1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Eco1 RT, Eco1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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phSyn2 sfGFP-RNF152

Plasmid

#215374

Purpose

Lentiviral expression of sfGFP-tagged lysosome membrane protein RNF152 under the neuron-specific human synapsin promoter.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

RNF152 (RNF152 Human)

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C14A)-flag-tev-halo-his

Plasmid

#227159

Purpose

For T-REX experiments of CYP2A6 C14A mutant. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 14 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C82A)-flag-tev-halo-his

Plasmid

#227160

Purpose

For T-REX experiments of CYP2A6 C82A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+8-cyp-33e1(C295A)-flag-tev-halo-his

Plasmid

#227156

Purpose

For T-REX experiments of cyp-33e1 C295A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 295 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1(C439A)-flag-tev-halo-his

Plasmid

#227157

Purpose

For T-REX experiments of cyp-33e1 C439A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRECMYC-Tsp1

Plasmid

#240712

Purpose

pLAM12 vector backbone containing Tsp1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Tsp11 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Kva1

Plasmid

#240709

Purpose

pLAM12 vector backbone containing Kva1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Kva1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Hcl1

Plasmid

#240714

Purpose

pLAM12 vector backbone containing Hcl1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Hcl1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRECMYC-Eas1

Plasmid

#240710

Purpose

pLAM12 vector backbone containing Eas1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Eas1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pREC1010lac-Tsp1

Plasmid

#240698

Purpose

RSF1010 origin of replication plasmid containing Tsp1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Tsp1 RT, Tsp1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pREC1010lac-Hcl1

Plasmid

#240700

Purpose

RSF1010 origin of replication plasmid containing Hcl recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Hcl1 RT, Hcl1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pREC1010lac-Eco2

Plasmid

#240692

Purpose

RSF1010 origin of replication plasmid containing Eco2 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Eco2 RT, Eco2 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRECMYC-Csp1

Plasmid

#240711

Purpose

pLAM12 vector backbone containing Csp1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Csp1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRECMYC-Abe1

Plasmid

#240708

Purpose

pLAM12 vector backbone containing Abe1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Abe1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRECMYC-Ban1

Plasmid

#240707

Purpose

pLAM12 vector backbone containing Ban1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Ban1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pREC1010lac-Csp1

Plasmid

#240697

Purpose

RSF1010 origin of replication plasmid containing Csp1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Csp1 RT, Csp1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pREC1010-Tsp1

Plasmid

#240687

Purpose

RSF1010 origin of replication plasmid containing Tsp1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Tsp1 RT, Tsp1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pREC1010-Hcl1

Plasmid

#240689

Purpose

RSF1010 origin of replication plasmid containing Hcl recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Hcl1 RT, Hcl1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pREC1010-Ebu1

Plasmid

#240688

Purpose

RSF1010 origin of replication plasmid containing Ebu1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Ebu1 RT, Ebu1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pREC1010-Ban1

Plasmid

#240682

Purpose

RSF1010 origin of replication plasmid containing Ban1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Ban1 RT, Ban1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pREC1010-Csp1

Plasmid

#240686

Purpose

RSF1010 origin of replication plasmid containing Csp1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Csp1 RT, Csp1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pALN130

Plasmid

#239798

Purpose

Express mEGFP tagged mouse CENPT

Depositor

Iain Cheeseman, Ally Nguyen

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

CENPT (Cenpt Mouse)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

Moloney Murine Leukemia Virus (MMLV) LTR promoter

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMHE-MmBorealin-OLLAS

Plasmid

#237439

Purpose

Expresses mouse Borealin tagged with OLLAS at C-term; made for in vitro transcription (T7 promoter)

Depositor

Michael Lampson

Article

Dudka et al Nature. 2025 Feb;638(8051):814-822. doi:

Insert

Borealin (Cdca8 Mouse)

Tags

OLLAS

Expression

Mammalian

Promoter

T7

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas3-CDA1

Plasmid

#229534

Purpose

pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

Depositor

Kevin Verstrepen

Article

Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

Inserts

Cas3
Cytidine deaminase
Uracil glycosylase inhibitor

Tags

XTEN

Expression

Bacterial and Yeast

Mutation

codon optimized for S.cerevisiae and codon optimi…

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS-9YS-miRFP670-TRF1

Plasmid

#227381

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric FUS IDR, 9YS (TERF1 Human)

Use

Lentiviral

Mutation

9YS

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS-27YS-miRFP670-TRF1

Plasmid

#227383

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric FUS IDR, 27YS (TERF1 Human)

Use

Lentiviral

Mutation

27YS

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS-15YS-miRFP670-TRF1

Plasmid

#227382

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric FUS IDR, 15YS (TERF1 Human)

Use

Lentiviral

Mutation

15YS

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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