We narrowed to 9,608 results for: FIE

1. pCIneoLuc-PP2A

   Plasmid

   #128349

   Purpose

   Vector for LUMIER assay

   Depositor

   Yutaka Hata

   Article

   Kawano et al J Biochem. 2015 May 14. pii: mvv051.

   Insert

   PP2A (PTPA Human)
   

   Use

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-sgRELA

   Plasmid

   #83944

   Purpose

   Lentiviral vector expressing an sgRNA targeting RELA NOTE: This plasmid does NOT contain Cas9.

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   sgRELA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSFDuet-1 IntS11 AA 300-597

   Plasmid

   #199329

   Purpose

   Expresses His-tagged Drosophila IntS11 amino acids 300-597

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   Integrator subunit 11 (IntS11 Fly)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   amino acids 300-597 only

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-E3CY56

   Plasmid

   #103075

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMB41

   Plasmid

   #73087

   Purpose

   N-terminal Avi-2xTEV-GFP vector, for fosmid recombineering

   Depositor

   Mike Boxem

   Article

   Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

   Insert

   Avi-GFP tag
   

   Use

   Tags

   Avi and GFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223-MCU-S259A

   Plasmid

   #31728

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   MCU (MCU Human)
   

   Use

   Pdonr gateway

   Tags

   None

   Expression

   Mutation

   Changed S259 to Alanine

   Promoter

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

7. mCherry-PHR-iSH-T2A-CIBNcaax

   Plasmid

   #173860

   Purpose

   Mammalian expression of mCherry-PHR-iSH-T2A-CIBNcaax (opto-PI3K)

   Depositor

   Takao Nakata

   Article

   Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

   Insert

   mCherry-PHR-iSH-T2A-CIBNcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pv9_CBX7_2xChromo-13XL-BASU

   Plasmid

   #179413

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.2xChromo-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7.2xChromo-13XL-BASU
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. F11-R pEBio

   Plasmid

   #61486

   Purpose

   Expresses full-length F11R (JAM1) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag and biotinylation sequence

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   F11R (F11R Human)
   

   Use

   Tags

   enzymatic biotinylation sequence and rat CD4 d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. BCL9-BirA*

    Plasmid

    #109147

    Purpose

    To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

    Depositor

    Mariann Bienz

    Article

    van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

    Insert

    BCL9 (BCL9 Human)
    

    Use

    Tags

    BirA(R118G)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-CDC42-DN

    Plasmid

    #109584

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative CDC42

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    CDC42 (cdc42 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    dominant negative

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMB72

    Plasmid

    #73088

    Purpose

    C-terminal GFP-2xTEV-Avi vector, for fosmid recombineering

    Depositor

    Mike Boxem

    Article

    Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

    Insert

    GFP-Avi tag
    

    Use

    Tags

    GFP and Avi

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. VIPR1

    Plasmid

    #51865

    Purpose

    Expresses full-length Vasoactive intestinal polypeptide receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    VIPR1 (VIPR1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-E6MBW6

    Plasmid

    #103102

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST40-2XFL-Src-Y530EEI

    Plasmid

    #140323

    Purpose

    Expresses Flag-tagged human Src-Y530EEI in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Y530EEI

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. 303Gal-FUS

    Plasmid

    #29614

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 18, 2011

    Availability

    Academic Institutions and Nonprofits only

17. SCUBE1-bio-His

    Plasmid

    #53415

    Purpose

    Expresses full-length Signal peptide, CUB and EGF-like domain-containing protein 1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SCUBE1 (SCUBE1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-CAG-DIO-erHRP(N175S mutant)

    Plasmid

    #79906

    Purpose

    Adeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)

    Depositor

    Joshua Sanes

    Article

    Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

    Insert

    erHRP(N175S mutant)
    

    Use

    AAV and Cre/Lox

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    N175S mutation

    Promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-uTEV1-MAVS-mTA4

    Plasmid

    #171002

    Purpose

    HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[Y536delinsFIVLI]

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMB71

    Plasmid

    #73085

    Purpose

    Expresses C. elegans optimized BirA under control of the intestinal elt-2 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

    Insert

    BirA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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