We narrowed to 8,345 results for: construct

1. pAMP-CY1_NF1 T2 R1276P (+minintr, KDR)

   Plasmid

   #119203

   Purpose

   transfer vector

   Depositor

   Helen Morrison

   Article

   Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

   Insert

   NF1 type 2 mini-gene (NF1 Human)
   

   Use

   Transfer vector

   Tags

   Expression

   Mutation

   R1276P

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe-Kras G15A

   Plasmid

   #58898

   Purpose

   Expresses human Kras with a G15A mutation

   Depositor

   Channing Der

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G15A mutation

   Promoter

   Available since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pLenti-EF1a-KLF4

   Plasmid

   #35389

   Depositor

   Feng Zhang

   Article

   Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

   Insert

   TALE-Klf4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2012

   Availability

   Academic Institutions and Nonprofits only

4. NAT-TET-OFF -LEU- 4xFLAG

   Plasmid

   #182527

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   7 Copies of TetO Box
   

   Use

   Tags

   4XFLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB3C5-proD-B0032-E0051

   Plasmid

   #107241

   Purpose

   Expresses lacZa-GFP fusion from constitutive proD promoter

   Depositor

   Joseph Davis, Robert Sauer

   Article

   Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

   Insert

   ProA-RBS(B0032)-Gemini (E0051)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   proD

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEnt L1L3 EF1a-tTA-3

   Plasmid

   #24415

   Depositor

   Edward Hsiao

   Article

   Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSET FLIPglu-170nDelta13

   Plasmid

   #13562

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16F Delta13
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   WT binding protein (no F16A)

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR puroR R4-R3

   Plasmid

   #43965

   Depositor

   Peter Hohenstein

   Article

   Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

   Insert

   puroR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSGV Rh Acceptor

   Plasmid

   #64271

   Purpose

   Retroviral golden gate cloning vector for expression of functional Rhesus Macaque T-Cell Receptor genes

   Depositor

   David Ott

   Article

   Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TDGS50

    Plasmid

    #183189

    Purpose

    TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

    Depositor

    Hsiao-Chun Huang

    Article

    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

    Inserts

    DivIVA-GFP(tevR M0050)
    TEV-S219V
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTK-EdnrbE2-Citrine

    Plasmid

    #127770

    Purpose

    Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Citrine activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    ednrbE2
    

    Use

    Enhancer reporter construct

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBW-m4046464

    Plasmid

    #148970

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. p3E-CMVmin:GFP no-pA

    Plasmid

    #80819

    Purpose

    CMVmin-promoted GFP without pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    p3E-CMV-GFP no pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p3E-GFPmem no-pA

    Plasmid

    #80817

    Purpose

    GFPmem without pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    GFPcaax no pA p3E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFP

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETDuet-PcPT-ximD-ximE

    Plasmid

    #172654

    Purpose

    Production of decursinol in E. coli

    Depositor

    Minjuan Xu

    Article

    Bu et al ACS Synth Biol. 2020 Sep 18;9(9):2282-22

    Insert

    PcPT, XimD and XimE
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-EF1a-OCT4

    Plasmid

    #35391

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    TALE-Oct4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pGBW-m4046574

    Plasmid

    #148976

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pTH848-gst-disec

    Plasmid

    #89589

    Purpose

    Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase codon-dis-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pME-mKate2 no stop

    Plasmid

    #80806

    Purpose

    red fluorophore without stop codon

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    pME-mKate2 no stop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCGN R-Ras 41A

    Plasmid

    #14730

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

    Insert

    R-ras (RRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    41A

    Promoter

    Available since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only