We narrowed to 30,517 results for: ide

1. pcDNA3.1-SLC25A46[I349D]-P2A-Electra1 (pAH13)

   Plasmid

   #227618

   Purpose

   Overexpression of SLC25A46 with I349D mutation in mammalian cell lines

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Use

   Tags

   P2A-mElectra1

   Expression

   Mammalian

   Mutation

   I349D

   Promoter

   CMV, T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)

   Plasmid

   #227619

   Purpose

   Overexpression of SLC25A46 lacking its N-terminal region in mammalian cell lines

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Use

   Tags

   P2A-mElectra1

   Expression

   Mammalian

   Mutation

   deleted aa 2-83

   Promoter

   CMV, T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKT-CNP

   Plasmid

   #211803

   Purpose

   Expresses 2',3'-cyclic nucleotide phosphodiesterase catalytic domain

   Depositor

   Emily Weinert

   Article

   Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

   Insert

   2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tet

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)

   Plasmid

   #195054

   Purpose

   integration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterization

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   NAT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLDP1

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)

   Plasmid

   #195053

   Purpose

   integration cassette for ku70 deletion and expression of NAT gene under pFBA for characterization

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   NAT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pFBA

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. ku70 insUP+pXYL+NAT+tNOS+ku70 insD (SBE178)

   Plasmid

   #195052

   Purpose

   integration cassette for ku70 deletion and expression of NAT gene under pXYL for characterization

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   NAT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pXYL

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)

   Plasmid

   #195051

   Purpose

   integration cassette for ku70 deletion and expression of NAT gene under pGPD for characterization

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   NAT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pGPD

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ku70 insUP+pADH2+NAT+tNOS+ku70 insD (SBE174)

   Plasmid

   #195050

   Purpose

   integration cassette for ku70 deletion and expression of NAT gene under pADH2 for characterization

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   NAT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pADH2

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. rUBR5_2383-2442_pGEX6P1

   Plasmid

   #209791

   Purpose

   Bacterial expression of UBR5 Mlle domain

   Depositor

   Kalle Gehring

   Article

   Lim et al J Biol Chem. 2006 May 19;281(20):14376-8

   Insert

   Mlle domain of UBR5 (Ubr5 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 2383-2442 only

   Promoter

   tac

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PET-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207387

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PET-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207382

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. B-HiFi SpCas9

    Plasmid

    #207362

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HiFi SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R691A, amino acids 1005-1013 replaced with two gl…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PET-HeFSpCas9-NLS-6xHis

    Plasmid

    #207391

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HeFSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    T7

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PET-B-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207378

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A, amino acids 1005-1013 replaced with two gl…

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PET-Blackjack SpCas9-NLS-6xHis

    Plasmid

    #207375

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Blackjack SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    amino acids 1005-1013 replaced with two glycine

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSUMO ACD11

    Plasmid

    #170738

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

    Insert

    acd11 (ACD11 Mustard Weed)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p35S_EVDwt

    Plasmid

    #167119

    Purpose

    Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) under CaMV 35S promoter.

    Depositor

    Arturo Marí-Ordóñez, Olivier Voinnet

    Article

    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

    Insert

    EVADE (EVD) (AT5G17125 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. RAP2A-p2A-E2Crimson

    Plasmid

    #83940

    Purpose

    Lentiviral vector expressing RAP2A-p2A-E2Crimson

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    p2A-E2Crimson

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. SLC35B2-p2A-E2Crimson

    Plasmid

    #83941

    Purpose

    Lentiviral vector expressing SLC35B2-p2A-E2Crimson

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Use

    Lentiviral

    Tags

    p2A-E2Crimson

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-sgALCAM

    Plasmid

    #83929

    Purpose

    Lentiviral vector expressing an sgRNA targeting ALCAM. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgALCAM
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge