We narrowed to 7,026 results for: ust

1. pLV.U6.gDNMT1.Cas9-T2A-GFP

   Plasmid

   #170362

   Purpose

   Plasmid used for Crispr/cas9 based disruption of human DNMT1.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

   Insert

   Cas9-T2A-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-NFIB-T2A-miRFP670

   Plasmid

   #187222

   Purpose

   Expresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   Nuclear Factor I B (NFIB Human)
   

   Use

   Tags

   Inserted T2A-miRFP670 at 3' terminal of MCS.

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-HSF1Δ∼PRD

   Plasmid

   #71725

   Purpose

   plasmid expressing mic-his tag phosphorylation-deficient hHSF1

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   phosphorylation-deficient HSF1 (HSF1 Human)
   

   Use

   Tags

   mac 6xHIS

   Expression

   Mammalian

   Mutation

   15 phosphorylation sites in RD mutated to Alanine

   Promoter

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Yiplac211 proGAL1-SZ1-RGG-GFP-RGG-RGG

   Plasmid

   #176396

   Purpose

   Expresses GFP tagged triple RGG scaffold with N-terminal SynZip1 coiled coil (Sz1). Expression induced in media containing galactose.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   GAL1 promoter, SZ1-RGG-GFP-RGG-RGG
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP in pDBEcR-pIND(SP1)-DD-W/Bla

   Plasmid

   #201560

   Purpose

   Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR and EGFP
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and pIND(SP1)

   Available since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR P2R-P3-EGFP

   Plasmid

   #48349

   Purpose

   Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Tomas Santalucia, Anna Planas

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Aminoacid 40 is valine in our sequence, while it …

   Promoter

   none

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAdCMV/V5-DEST-STAT3-3xFlag

   Plasmid

   #99260

   Purpose

   Adenoviral vector encoding Flag-tagged murine STAT3

   Depositor

   Alejandro Adam

   Article

   Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

   Insert

   Signal transducer and activator of transcription 3 (Stat3 Mouse)
   

   Use

   Adenoviral

   Tags

   3x-Flag

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9-beta-mmr

   Plasmid

   #169241

   Purpose

   Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   mutL-E36K
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed glutamic acid 36 to lysine

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ana GvpC-SpyTag-His in pET28a

   Plasmid

   #85737

   Purpose

   Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (fused to SpyTag and His-tagged)
   

   Use

   Tags

   His-tag and SpyTag

   Expression

   Bacterial

   Mutation

   Added an extra glycine after the methionine at th…

   Promoter

   T7

   Available since

   March 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. A*03:02:01

    Plasmid

    #203294

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*03:02:01 (HLA-A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. GlycophorinA-CD4d3+4-bio

    Plasmid

    #73093

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinA with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Glycophorin A (GYPA Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. B*58:02:01

    Plasmid

    #203262

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*58:02:01 (HLA-B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. A*66:01:01

    Plasmid

    #203291

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*66:01:01 (HLA-A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lactotransferrin-CD4d3+4-bio

    Plasmid

    #73124

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Lactotransferrin with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Lactotransferrin (LTF Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-iPEmax-Hygro

    Plasmid

    #214020

    Purpose

    inducible PEmax system for controllable prime editing; this plasmid is used to insert PEmax prime editor in one allele of AAVS1 locus

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PEmax
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEN25-Ub Hs

    Plasmid

    #83232

    Purpose

    pHis-par2-Ub Hs

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    Ubiqutin (UBC Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. B1 Integrin-GFP

    Plasmid

    #186138

    Purpose

    For expression of recombinant beta1 integrin cytosolic domain with C-terminal GFP tag in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Tags

    MBP and mEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHS1232

    Plasmid

    #205969

    Purpose

    MWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promoters

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas5-HNH proteins and CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac (proteins), pJ23119 (crRNA)

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHS1801

    Plasmid

    #205966

    Purpose

    JAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promoters

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH proteins and CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac (proteins), pJ23119 (crRNA)

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. p2T-CMV-miniSdd6-BE4max-BlastR

    Plasmid

    #204850

    Purpose

    For cytosine base editing using miniSdd6 in mammalian cells

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd6-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV, SV40, EM7

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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