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pAAV-EFS-CasRx-T2A-mCherry-pA

Plasmid

#192494

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DjCas13d-pA

Plasmid

#192495

Purpose

To express DjCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DIO-DjCas13d-pA

Plasmid

#192498

Purpose

To express DjCas13d in a Cre dependent manner

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-CasRx-pA

Plasmid

#192482

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-0.4CaMKII-CasRx-T2A-mCherry-pA

Plasmid

#192483

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

0.4CaMKIIa

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CasRx-T2A-mCherry-pA

Plasmid

#192484

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

Ef1a

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-CasRx-pA

Plasmid

#192487

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C82A)-flag-tev-his

Plasmid

#227166

Purpose

For overexpression of CYP2A6 C82A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1-flag-tev-halo-his

Plasmid

#227151

Purpose

For T-REX experiments of cyp-33e1. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6-flag-tev-halo-his

Plasmid

#227154

Purpose

For T-REX experiments of CYP2A6. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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rAAV-Ef1α-EGFP-WPRE-ID-PolyA

Plasmid

#241829

Purpose

AAV construct for constitutive expression of EGFP. Contains a molecular barcode for identification of AAV genome using single-cell sequencing.

Depositor

Benjamin Arenkiel

Article

Belfort et al Front Neurosci. 2025 Feb 13;19:1531122.

Insert

EGFP-WPRE-ID-PolyA

Use

AAV

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-p53-G609T

Plasmid

#229531

Purpose

Expresses p53 mutant CASM203 in mamallian cells

Depositor

Robin Fåhraeus

Article

Sajwan et al Br J Cancer. 2025 Jul 26. doi: 10.1038/s

Insert

p53-CDS (TP53 Human)

Expression

Mammalian

Mutation

c.G609T

Promoter

CMV

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-p53-G609A

Plasmid

#229532

Purpose

Expresses p53 mutant G609A in mamallian cells

Depositor

Robin Fåhraeus

Article

Sajwan et al Br J Cancer. 2025 Jul 26. doi: 10.1038/s

Insert

p53-CDS (TP53 Human)

Expression

Mammalian

Mutation

c.G609A

Promoter

CMV

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-p53-G609C

Plasmid

#229533

Purpose

Expresses p53 mutant G609C in mamallian cells

Depositor

Robin Fåhraeus

Article

Sajwan et al Br J Cancer. 2025 Jul 26. doi: 10.1038/s

Insert

p53-CDS (TP53 Human)

Expression

Mammalian

Mutation

c.G609C

Promoter

CMV

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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ER-frNFAST

Plasmid

#233599

Purpose

Expression of frNFAST on the ER membrane

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

frNFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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PM-frNFAST

Plasmid

#233602

Purpose

Expression of frNFAST on the PM facing the cytosol

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

frNFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FRB-Fis1 (no mt-mKeima)

Plasmid

#223795

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FRB-Fis1

Use

Lentiviral

Tags

FRB

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-hfCas13d-pa

Plasmid

#233037

Purpose

To express hfCas13d from a EF1a promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d

Use

AAV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MfnG-EcYRS-EGFP*

Plasmid

#191119

Purpose

A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein

Tags

His tag

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2

Plasmid

#221082

Purpose

Plasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

ACC-msfGFP[r5M]_ACC-mEBFP2

Use

Synthetic Biology

Promoter

CMV, T7

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR_NPM2-T2A-mGreenLantern-PuroTK

Plasmid

#222910

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to NPM2.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR_FIGLA-T2A-mGreenLantern-PuroTK

Plasmid

#222905

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to FIGLA.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR2_TFAP2C-T2A-mGreenLantern-Hygro

Plasmid

#222915

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to TFAP2C.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ZGLP1

Plasmid

#222926

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ZGLP1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

ZGLP1 (ZGLP1 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-SOHLH1

Plasmid

#222922

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

SOHLH1 (SOHLH1 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)

Plasmid

#208869

Purpose

For mammalian expression of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

HA

Expression

Mammalian

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-GST-TEV-GFP-NAP1

Plasmid

#208870

Purpose

For expression in E. coli of GFP-NAP1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

GFP and GST-TEV

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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SF3B3

Plasmid

#156003

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SF3B3 (SF3B3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

S6L

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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RBMX2-1

Plasmid

#155915

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBMX2 (RBMX2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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PHF6

Plasmid

#155821

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PHF6 (PHF6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge