We narrowed to 23,609 results for: Sele

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PET-HeFSpCas9-NLS-6xHis

Plasmid

#207391

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HeFSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-HiFi SpCas9-NLS-6xHis

Plasmid

#207378

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A, amino acids 1005-1013 replaced with two gl…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PET-Blackjack SpCas9-NLS-6xHis

Plasmid

#207375

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Blackjack SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 1005-1013 replaced with two glycine

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IRES-puro-3xFLAG

Plasmid

#204545

Purpose

Expresses 3xFLAG tag in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

3xFlag

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-6xHis-TEV-ORF9c

Plasmid

#201023

Purpose

Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

open reading frame 9c

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-mut4/1_2_VB

Plasmid

#186444

Purpose

Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Mutation

Cas4 domain (K81A)

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-Hs4EHP_52-234opt_AE

Plasmid

#148566

Purpose

Bacterial Expression of Hs4EHP_52-234opt

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_52-234opt (EIF4E2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-52-GB1_AE

Plasmid

#148552

Purpose

Bacterial Expression of HsGIGYF1_33-52

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-52 (GIGYF1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-71_AE

Plasmid

#148553

Purpose

Bacterial Expression of HsGIGYF1_33-71

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-71 (GIGYF1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-71-GB1_AE

Plasmid

#148554

Purpose

Bacterial Expression of HsGIGYF1_33-71

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-71 (GIGYF1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-103-E44AE45FQ87A_AE

Plasmid

#148555

Purpose

Bacterial Expression of HsGIGYF1_33-103-E44AE45FQ87A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-103-E44AE45FQ87A (GIGYF1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-103-GB1_AE

Plasmid

#148557

Purpose

Bacterial Expression of HsGIGYF1_33-103

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-103 (GIGYF1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(103)-GFP

Plasmid

#197890

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(553)-GFP

Plasmid

#197888

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(285)-GFP

Plasmid

#197889

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_PM

Plasmid

#182813

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_cyto

Plasmid

#182814

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iFluoxSnFR_ER

Plasmid

#182815

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_ER

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iFluoxSnFR_PM

Plasmid

#182816

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_PM

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iFluoxSnFR_cyto

Plasmid

#182817

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_cyto

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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