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E3_pENTR_L3-L2_HD_G1333-DddtoxA-N

Plasmid

#191590

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_NI_G1333-DddtoxA-N

Plasmid

#191591

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_NN_G1333-DddtoxA-N

Plasmid

#191592

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_HD_G1397-DddtoxA-C

Plasmid

#191593

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E3_pENTR_L3-L2_NI_G1397-DddtoxA-C

Plasmid

#191594

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E3_pENTR_L3-L2_NN_G1397-DddtoxA-C

Plasmid

#191595

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_HD_G1333-DddtoxA-C

Plasmid

#191579

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_NG_G1333-DddtoxA-C

Plasmid

#191580

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_HD_G1333-DddtoxA-N

Plasmid

#191581

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_NG_G1333-DddtoxA-N

Plasmid

#191582

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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LV-sgRNA-mCherry-Puro sgPIDD

Plasmid

#211528

Purpose

Deletes PIDD

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgPIDD

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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BPK1520 sgRPS3

Plasmid

#211526

Purpose

Facilitate knock-in of RPS3-Keima fusion protein

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgRPS3

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 hygro sgUCK1

Plasmid

#211524

Purpose

Deletes UCK1

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUCK1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMOD_A-AtUBI10-dCas9-24XGP41

Plasmid

#202011

Purpose

Moclo level 1 - Module A, Promoter: AtUBI10, Gene: dCas9-24XGP41, Terminator: HSP

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Insert

dCas9-24XGP41

Use

Synthetic Biology; Moclo level 1 vector

Promoter

AtUBI10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pJFRC7-JEDI-1P

Plasmid

#202618

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P under the promoter hsp70 for Drosophila (insect) expression

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Expression

Insect

Promoter

hsp70

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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Derp2.pJL1

Plasmid

#199116

Purpose

In vitro expression of Der p 2, a major common allergen from Dermatophagoides pteronyssinus (dust mite), with synthetic site for glycosylation encoded (GGNWTT)

Depositor

Michael Jewett

Article

Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102

Insert

Der p 2 dust mite allergen

Tags

Glyctag, His-tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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ptRNAfMet-A37U

Plasmid

#196659

Purpose

Plasmid for overexpression of mutated initiator tRNA (A37U)

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

tRNAfMet-A37U expression cassette

Expression

Bacterial

Mutation

A37U in tRNAfMet

Promoter

metY native promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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ptRNAfMet-A37G

Plasmid

#196658

Purpose

Plasmid for overexpression of mutated initiator tRNA (A37G)

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

tRNAfMet-A37G expression cassette

Expression

Bacterial

Mutation

A37G in tRNAfMet

Promoter

metY native promoter

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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QUAS-0-T7-TetO-GFP_ColE1

Plasmid

#171662

Purpose

QUAS directly upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli. Contains the ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-T7-TetO-GFP ssrA-T7 Stop

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL8

Plasmid

#176184

Purpose

gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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