We narrowed to 23,721 results for: sequence
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pSTAP-seq_fly-tj
Plasmid#86386PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster tj enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
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p252 pPr-CREM/CMV-STOP-luc
Plasmid#8406PurposeExpression of modified Cre from androgen-inducible probasin promoter and LOX-dependent conditional reporter cassette.DepositorInsertCREM/lox/luciferase
UseCre/LoxTagsExpressionMammalianMutationmodified CrePromoterProbasin promoter, CMVAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
jk65
Plasmid#87019Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsSNAP-Nlg1AB
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCALNL_Ch10
Plasmid#81224PurposepCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in PCDH15 locus on chromosome 10DepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag‐Tim2
Plasmid#49213Purposeexpresses Flag tagged Tim2DepositorInsertTim2 (Timd2 Mouse)
UseTagsCD8 signal sequence and FLAGExpressionMammalianMutationno Tim2 signal sequence; R80L and S196D compared …PromoterEF1aAvailable sinceDec. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
MET FLAG46QDProCFPp303
Plasmid#15589DepositorInserthtt 46Q Delta Pro (HTT Human)
UseTagsCFP and FLAGExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
GAL HA25Q+ProCFPp303
Plasmid#15570DepositorInserthtt 25Q + Pro (HTT Human)
UseTagsCFP and HAExpressionYeastMutationProline-rich region presentPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJAK184.tetR.PT5-3.dCas9
Plasmid#232482Purposeoptimized ahTet inducible dCas9 for insertion into C. difficile genomeDepositorInsertCatalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
UseCRISPRTagsExpressionBacterialMutationPromoterTetR-PT5-3Available sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJC.15A.sgRNA.TA
Plasmid#232483PurposesgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.DepositorInsertsToxin-antitoxin System from CD630
medium-copy-number p15A origin of replication
mCherry
UseCRISPRTagsExpressionBacterialMutationPromoterP3 constitutive promoter and native promoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFLtail
Plasmid#232055PurposeExpresses construct for surface tethering: R. norvegicus NFL tail domainDepositorInsertNeurofilament-Light tail domain
UseTags6xHisExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHtail
Plasmid#232057PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domainDepositorInsertNeurofilament-Heavy tail domain
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m1
Plasmid#204463PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR A127N mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR A127N mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m2
Plasmid#204464PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L128C mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR L128C mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m3
Plasmid#204465PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127S mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR L125W-A127S mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m4
Plasmid#204466PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR L125W-A127T mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m5
Plasmid#204467PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125F-A127G-S129N mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR L125F-A127G-S129N mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m6
Plasmid#204468PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-S129N-L130V mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR L125W-S129N-L130V mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m7
Plasmid#204469PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T-L128M mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR L125W-A127T-L128M mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only
