We narrowed to 9,329 results for: sgrna

1. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

   Plasmid

   #181791

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

   Plasmid

   #92121

   Purpose

   CRISPR-Cas9 based efficient genome editing in S. aureus

   Depositor

   Sheng Yang

   Article

   Liu et al Acta Biochim Biophys Sin (Shanghai). 201

   Insert

   Cas9-Pxyltet-sgRNA-pj23119
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1

   Plasmid

   #117405

   Purpose

   SpyCas9 sgRNA 1 targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   SpyCas9 sgRNA targeting TLR 2.0
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

   Plasmid

   #128749

   Purpose

   Expresses control gRNA

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40

   Plasmid

   #179117

   Purpose

   Expresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

   Depositor

   Baljit Khakh

   Article

   Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

   Insert

   Fermt2 sgRNA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-pMecp2-dSaCas9-VP160-pU6-sgRNA

   Plasmid

   #158987

   Purpose

   Vector D encodes pAAV-pMecp2-dSaCas9-VP160-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-VP160
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pMecp2

   Available since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

   Plasmid

   #86987

   Purpose

   For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP

   Plasmid

   #162337

   Purpose

   Lentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   S. pyogenes sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6; mU6

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP

   Plasmid

   #194283

   Purpose

   EF1a-dCas9-KRAB-GFP with Slc1a3 sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

    Plasmid

    #181788

    Purpose

    Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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