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pSpCas9 (PX165)

Plasmid

#48137

Purpose

Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pRDA_867

Plasmid

#231109

Purpose

A>G base editor

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

ABE8e-nCas9-SpG-HA

Use

CRISPR and Lentiviral

Mutation

D10A

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pNCS BFP

Plasmid

#91757

Purpose

Bacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)

Depositor

Erik Rodriguez, Roger Tsien

Article

Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

Insert

Blue Fluorescent Protein

Tags

Hexa-Histidine, Xpress epitope for detection with…

Expression

Bacterial

Mutation

GFP (Y66H)

Promoter

T7

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-FLAG-FAM134B

Plasmid

#128260

Purpose

Expresses FAM134B in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

FAM134B (RETREG1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn1alpha

Plasmid

#58266

Purpose

Expression plasmid for HA-tagged rat Neurexin 1alpha

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn1alpha (Nrxn1 Rat)

Tags

HA

Expression

Mammalian

Mutation

see comments

Promoter

CAG

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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EGFPC1-huNFATc1EE-WT

Plasmid

#24219

Depositor

Jerry Crabtree

Article

Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

Insert

NFATc1 (NFATC1 Human)

Tags

EE and GFP

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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tubP-FRT>GAL80-6-FRT>

Plasmid

#63173

Purpose

ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"Flp-Out" GAL80

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pP3-GFP

Plasmid

#53436

Purpose

Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

P3 promoter

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

none

Promoter

P3

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pRDB_092

Plasmid

#231110

Purpose

C>T base editor

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

TadA-CDd-nCas9-SpG-UGI

Use

CRISPR and Lentiviral

Mutation

D10A

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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TAF3 PHD_pGEX-6P-2

Plasmid

#92100

Purpose

Expresses GST fusion TAF3 PHD domain protein

Depositor

Albert Jeltsch

Article

Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

Insert

TAF3 PHD domain (TAF3 Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR

Plasmid

#13507

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-Merlin

Plasmid

#84293

Purpose

Expression of wt Merlin

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Merlin (NF2) (NF2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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phL1A-pcDNA3

Plasmid

#12307

Depositor

Vance Lemmon

Article

Hlavin et al Genomics. 1991 Oct . 11(2):416-23.

Insert

L1CAM (L1CAM Human)

Expression

Mammalian

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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EMTB-3XGFP

Plasmid

#26741

Depositor

William Bement

Article

Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

Insert

ensconsin (MAP7 Human)

Use

Probe for microtubules

Tags

3x EGFP and Myc

Expression

Mammalian

Mutation

Only contains the microtubule binding-domain of e…

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Mmp11

Plasmid

#209520

Purpose

Expresses luciferase under control of Mmp11 promoter in transfected cells

Depositor

Jennifer Mitchell

Article

Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

Insert

Mmp11 promoter (Mmp11 Mouse)

Use

Luciferase

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-NLS-mCherry

Plasmid

#231890

Purpose

mCherry-tagged nuclear rupture marker

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

mCherry-SV40 NLS-mCherry

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYES2-HTH-HHT2

Plasmid

#86482

Purpose

yeast expression of HHT2 with His10-TEV-HA tag

Depositor

Akash Gunjan

Article

Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

Insert

HHT2 (HHT2 Budding Yeast)

Tags

HIS10-TEV-HA (HTH)

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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EMTB-mCherry

Plasmid

#26742

Depositor

William Bement

Article

Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

Insert

ensconsin (MAP7 Human)

Use

Probe for microtubules

Tags

2x mCherry and Myc

Expression

Mammalian

Mutation

Microtubule binding-domain of ensconsin (amino ac…

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 sgSp1

Plasmid

#174907

Purpose

Second generation sgRNA against Sp1

Depositor

Jane Clifford

Article

Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

Insert

Specificity Protein 1 (SP1 Human)

Expression

Mammalian

Mutation

Missense mutation to mutate PAM sequence

Promoter

EFS-NS

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

Plasmid

#188703

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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