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All-in-one lenti-BE4-NG

Plasmid

#200448

Purpose

Lentivirus vector for base editing

Depositor

Benjamin Cravatt

Publication

Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_eZ3_Gal4VP64

Plasmid

#169917

Purpose

Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

Depositor

Zhili Rong

Publication

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch3-GL4VP64

Use

Lentiviral

Expression

Mammalian

Mutation

Additional EGF repeat between the extracellular d…

Promoter

PGK

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgAMPKa1

Plasmid

#138684

Purpose

Expresses a human AMPKa1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Publication

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgAMPKa1 human (PRKAA1 Human)

Use

Lentiviral

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLXSN p200 CUX1

Plasmid

#90470

Purpose

Retroviral vector expressing human p200 CUX1 with a Myc and HA tag at the N- and C-terminus, respectively

Depositor

Alain Nepveu

Publication

Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

Insert

CUX1 (CUX1 Human)

Use

Retroviral

Tags

Ha and myc

Expression

Mammalian

Promoter

Moloney murine leukemia virus long terminal repeat

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB3051(gRNA X-3 XI-2 XII-2)

Plasmid

#73293

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFB2G

Plasmid

#195290

Purpose

Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection

Depositor

Scott Lowe

Publication

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mTagBFP

Expression

Mammalian

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-GFP

Plasmid

#122100

Purpose

AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has service

AAV2

Insert

GFP

Use

AAV

Tags

NA

Expression

Mammalian

Promoter

EF1a（1.1kb short version)

Available since

May 30, 2020

Availability

Academic Institutions and Nonprofits only

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pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Plasmid

#78607

Purpose

A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Publication

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

EF1a-short; U6

Available since

Sept. 16, 2017

Availability

Academic Institutions and Nonprofits only
pMS13

Plasmid

#229474

Purpose

Expresses RssB with Q254A & N256A mutation

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (Q254A, N256A)

Expression

Bacterial

Mutation

Q254A, N256A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS19

Plasmid

#229475

Purpose

Expresses RssB with E135A, E136A, & E137A mutation

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF42

Plasmid

#229467

Purpose

Expresses RssB with S29C & E229C mutation

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (S29C, E229C)

Expression

Bacterial

Mutation

S29C, E229C

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS21

Plasmid

#229476

Purpose

Expresses RssB with E135A, E136A, & E137A mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Tags

10xHis

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS26

Plasmid

#229471

Purpose

Expresses RssB with D104N mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D104N)

Tags

10xHis

Expression

Bacterial

Mutation

D104N

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS23

Plasmid

#229470

Purpose

Expresses RssB with D104A mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D104A)

Tags

10xHis

Expression

Bacterial

Mutation

D104A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS18

Plasmid

#229462

Purpose

Expresses IraD with Q68A mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (Q68A)

Tags

6xHis

Expression

Bacterial

Mutation

Q68A

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMS12

Plasmid

#229461

Purpose

Expresses IraD with W64R mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (W64R)

Tags

6xHis

Expression

Bacterial

Mutation

W64R

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF40

Plasmid

#229459

Purpose

Expresses IraD with R70A mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (R70A)

Tags

6xHis

Expression

Bacterial

Mutation

R70A

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF26

Plasmid

#229458

Purpose

Expresses IraD with Q84A mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (Q84A)

Tags

6xHis

Expression

Bacterial

Mutation

Q84A

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF25

Plasmid

#229457

Purpose

Expresses IraD with R67A mutation with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (R67A)

Tags

6xHis

Expression

Bacterial

Mutation

R67A

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF18

Plasmid

#229456

Purpose

Expresses IraD (aa17-130) with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (aa 17-130 only)

Tags

6xHis

Expression

Bacterial

Mutation

aa 17-130 only

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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