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pcDNA5/FRT/TO V5 HSPA1L

Plasmid

#19511

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 1-like (HSPA1L Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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Matrix-roGFP

Plasmid

#49437

Purpose

Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells

Depositor

Paul Schumacker

Article

Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

Insert

Matrix-roGFP2

Use

Adenoviral

Tags

Cytochrome c oxidase subunit IV mitochondrial tar…

Expression

Mammalian

Mutation

C48S, Q80R, S147C, and Q204C in Clontech's G…

Promoter

CMV

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-METTL14

Plasmid

#53740

Purpose

For expression of METTL14 (methyltransferase-like 14) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Methyltransferase-like 14 (METTL14 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsEDC3

Plasmid

#25032

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

EDC3 (EDC3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pMRXIP VPS16-GFP

Plasmid

#67023

Purpose

Human VPS16 ORF was inserted into pMRXIP GFP-N vector

Depositor

Noboru Mizushima

Article

Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

Insert

Homo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (VPS16 Human)

Use

Retroviral

Tags

GFP

Promoter

CMV

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only
pClneoMyc human CIN85

Plasmid

#47935

Purpose

expresses Myc tagged human CIN85 in mammalian cells

Depositor

Yutaka Hata

Article

Kawata et al J Biochem. 2006 May;139(5):931-9.

Insert

CIN85 (SH3KBP1 Human)

Tags

Myc

Expression

Mammalian

Mutation

contains amino acids 2-665 of human CIN85; D114G

Promoter

CMV

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-JunWT-Myc

Plasmid

#47443

Purpose

mammalian expression of WT c-Jun

Depositor

Axel Behrens

Article

Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

Insert

Jun (Jun Mouse)

Tags

Flag and myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pJMP2

Plasmid

#79874

Purpose

Bacillus subtilis sgRNA expression vector; integrates into amyE

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

sgRNA RR1

Use

CRISPR

Expression

Bacterial

Promoter

veg

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB3alpha2_35S:P19:Tnos (GB1203)

Plasmid

#68214

Purpose

Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

P19 silencing suppressor

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-MED17

Plasmid

#15410

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED17 (MED17 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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GPD-roGFP

Plasmid

#49436

Purpose

Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells

Depositor

Paul Schumacker

Article

Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

Insert

GPD-roGFP2

Use

Adenoviral

Tags

Glycerol phosphate dehydrogenase, 5' to roGF…

Expression

Mammalian

Mutation

C48S, Q80R, S147C, and Q204C in Clontech's G…

Promoter

CMV

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pBGPa-CMV-GFP-OSBP PH domain

Plasmid

#58724

Purpose

Expresses GFP-Tagged OSBP PH domain in mammalian cells

Depositor

Tim Levine

Article

Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

Insert

Oxysterol-binding Protein PH domain (OSBP Human)

Tags

GFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
5' Del 1

Plasmid

#16049

Depositor

Robert Benezra

Article

Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

Insert

Id1 promoter, 5' deletion (Id1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

-1372 to +88 of promoter

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
5' Del 2

Plasmid

#16050

Depositor

Robert Benezra

Article

Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

Insert

Id1 promoter, 5' deletion (Id1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

-1147 to +88 of promoter

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pMJ922

Plasmid

#78312

Purpose

Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

Depositor

Martin Jinek

Article

Burger et al Development. 2016 Apr 29. pii: dev.13480

Insert

SpCas9

Tags

HA-2xNLS-EGFP-NLS and His6-MBP-TEV

Expression

Bacterial

Mutation

human codon-optimized gene sequence

Promoter

T7

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK

Plasmid

#25033

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

Rck (DDX6 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab1a S25N

Plasmid

#46777

Purpose

expression of S25N Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a S25N mutant (RAB1A Human)

Tags

myc

Expression

Mammalian

Mutation

S25N (dominant negative)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
HC078 (3xHA-Atg13)

Plasmid

#59544

Purpose

Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

Depositor

Daniel Klionsky

Article

Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

Insert

ATG13 (ATG13 Budding Yeast)

Tags

3xHA

Expression

Bacterial and Yeast

Mutation

AvrII site created after start codon

Promoter

ATG13

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
HA Sec13 pRK5

Plasmid

#46332

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Sec13 (SEC13 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

Plasmid

#35511

Purpose

AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T and T159C

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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