We narrowed to 4,430 results for: Erf

1. C105m

   Plasmid

   #121008

   Purpose

   MoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   sfYFP-AAV ssrA (has bbsI)
   

   Use

   Synthetic Biology

   Tags

   AAV degradation tag for ssrA

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. C106m

   Plasmid

   #121009

   Purpose

   MoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   sfYFP-DAS ssrA (has bbsI)
   

   Use

   Synthetic Biology

   Tags

   DAS degradation tag for ssrA

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTH339-SUP45(1-138)-GalBD

   Plasmid

   #29739

   Depositor

   Tobias von der Haar

   Insert

   SUP45 (SUP45 Budding Yeast)
   

   Use

   Tags

   Gal Activation domain and Myc

   Expression

   Yeast

   Mutation

   Codons 1-138 of the yeast SUP45 gene only

   Promoter

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. U6+27-20pf

   Plasmid

   #21978

   Depositor

   Phil Sharp

   Article

   Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

   Insert

   U6 miR-20 perfect
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

5. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

   Plasmid

   #192664

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, D839A, H840A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. XClone_Cas9_KRAB_T2A_BFP

   Plasmid

   #141208

   Purpose

   Tunable and temporal expression control of dCas9

   Depositor

   Rory Johnson

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOpen-HSlig3

   Plasmid

   #165574

   Purpose

   Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992))

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   DNA Ligase III
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. CMV-d2eGFP-20pf

   Plasmid

   #21971

   Depositor

   Phil Sharp

   Article

   Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

   Insert

   CMV d2eGFP miR-20 perfect
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

9. CMV-d2eGFP-16pf

   Plasmid

   #21969

   Depositor

   Phil Sharp

   Article

   Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

   Insert

   CMV d2eGFP miR-16 perfect
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

10. C114m

    Plasmid

    #121013

    Purpose

    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    dCas9 with no bbsI or BsaI sites
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTH341-SUP45(139-437)-GalBD

    Plasmid

    #29741

    Depositor

    Tobias von der Haar

    Insert

    SUP45 (SUP45 Budding Yeast)
    

    Use

    Tags

    Gal Activation domain and Myc

    Expression

    Yeast

    Mutation

    Codons 139-437 of the yeast SUP45 gene only

    Promoter

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTH342-SUP45(276-437)-GalBD

    Plasmid

    #29742

    Depositor

    Tobias von der Haar

    Insert

    SUP45 (SUP45 Budding Yeast)
    

    Use

    Tags

    Gal Activation domain and Myc

    Expression

    Yeast

    Mutation

    Codons 276-437 of the yeast SUP45 gene only

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Yeast Inducible CRISPRi Library

    Pooled Library

    #161829

    Purpose

    ATc inducbile yeast CRISPRi library

    Depositor

    Saeed Tavazoie

    Article

    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

    Expression

    Yeast

    Species

    Saccharomyces cerevisiae

    Use

    CRISPR

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

14. pLPC hRap1-FL

    Plasmid

    #12542

    Depositor

    Titia de Lange

    Article

    Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

    Insert

    Repressor Activator Protein 1 (TERF2IP Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pUC8 mouse IFN-gamma

    Plasmid

    #10955

    Depositor

    Howard Young

    Insert

    interferon-gamma (Ifng Mouse)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HA-p48

    Plasmid

    #11614

    Depositor

    Steven Johnson

    Insert

    Interferon-Stimulated Gene Factor 3 (IRF9 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

17. CMV-dCas9_puroR

    Plasmid

    #219825

    Purpose

    CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    CMV

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. J-IRF5-5

    Plasmid

    #166525

    Purpose

    scFv of a human scaffold targeting Interferon regulatory factor 5. Antigen coverage aa E232-L434 of 498

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. UKC1620-SUP35wt

    Plasmid

    #29730

    Depositor

    Tobias von der Haar

    Insert

    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Wild type gene including genomic sequence 955 bp …

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

20. PDZD3

    Plasmid

    #38880

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PDZD3 (PDZD3 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only