We narrowed to 3,703 results for: Gla

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36INS1-pBAD_GB

Plasmid

#78673

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [G:36INS1-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS1-pBAD_FB

Plasmid

#78672

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [F:36INS1-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS1-pBAD_EB

Plasmid

#78671

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [E:36INS1-pBAD:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pBAD Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Lf-MetQ

Plasmid

#78305

Purpose

Methionine Binding Protein (MetQ) with C-Terminal 6x Histidine Tag

Depositor

Douglas Rees

Article

Nguyen et al Biol Chem. 2015 Sep;396(9-10):1127-34. d

Insert

Methionine Binding Protein (metQ )

Tags

6x Histidine Tag

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-A

Plasmid

#64979

Purpose

Expresses the E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E108A

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-K

Plasmid

#64978

Purpose

Expresses the E105K mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pBS BMPR (DM#137)

Plasmid

#15059

Depositor

Douglas Melton

Article

Graff et al Cell. 1994 Oct 7. 79(1):169-79.

Insert

BMP receptor (bmpr1a Frog)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pSP64T3 BMP2 pro dnmVg1 (DM#182)

Plasmid

#15074

Depositor

Douglas Melton

Article

Thomsen et al Cell. 1993 Aug 13. 74(3):433-41.

Insert

BMP2-Vg1 dom neg

Use

Sp6 expression for use in xenopus

Mutation

dominant negative version of BMP2-Vg1 chimera. Th…

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
BS.YxiN.Twin1.a1-368.wt

Plasmid

#13528

Depositor

David McKay

Article

Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

Insert

B. subtilis DEAD-box helicase YxiN first two domains

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

deleted amino acid residues 369-479

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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BS.YxiN.Twin1.a404-479.wt

Plasmid

#13529

Depositor

David McKay

Article

Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

Insert

B. subtilis DEAD-box helicase YxiN specific RNA binding domain

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

delete amino acid residues 1-403

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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BS.YxiN.Twin1.a207-368.wt

Plasmid

#13531

Depositor

David McKay

Article

Caruthers et al Acta Crystallograph Sect F Struct Biol C

Insert

B. subtilis DEAD-box helicase YxiN second domain

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

deleted amino acid residues 1-206 and 369-479

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pRK793

Plasmid

#8827

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219V mutant

Tags

His, MBP (with TEV), and polyarginine

Expression

Bacterial

Mutation

S219V mutation (improves stability)

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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p3xFlag-Dam

Plasmid

#121909

Purpose

Expresses E. coli Dam methyltransferase containing an N-ternimal 3xFlag tag

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

Dam human codon optimized (dam E. coli)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGFP-Cytochrome C

Plasmid

#41181

Depositor

Douglas Green

Article

Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Insert

Cytochrome C (CYCS Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCdh2.1 GFP

Plasmid

#209099

Purpose

GFP expressing shRNA targeting Cdh2

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCdh2.1

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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human MMP-9 promoter

Plasmid

#53434

Purpose

promoter activity assay

Depositor

Etty Benveniste, Douglas Boyd

Article

Ma et al J Immunol. 2001 Nov 1;167(9):5150-9.

Insert

MMP-9 promoter (MMP9 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

MMP9

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-hnRNP-H

Plasmid

#23020

Depositor

Douglas Black

Article

Chou et al Mol Cell Biol. 1999 Jan . 19(1):69-77.

Insert

hnRNP-H (HNRNPH1 Human)

Tags

His

Expression

Bacterial

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

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pRK1035

Plasmid

#8832

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

TVMV protease

Tags

His and MBP (cleaved)

Expression

Bacterial

Mutation

synthetic gene with codons optimized for E. coli

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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RIP-Timer (DM#285)

Plasmid

#15109

Depositor

Douglas Melton

Article

Dor et al Nature. 2004 May 6. 429(6987):41-6.

Insert

insulin II promoter (Ins2 Rat)

Expression

Mammalian

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-6XHis-FLKSRP

Plasmid

#23001

Depositor

Douglas Black

Article

Hall et al Mol Biol Cell. 2004 Feb . 15(2):774-86.

Insert

KSRP (KHSRP Human)

Tags

EGFP and His

Expression

Mammalian

Mutation

valine inserted at aa#2

Available Since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

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