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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,310 results for: LEA

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Showing: 3081 - 3100 of 12310 results

  1. pOCC28

    Plasmid
    #118873
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and MBP, cleavab…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118873-plasmid-map-sequence-id-232344

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  3. pET24a-VHH-tev-std

    Plasmid
    #109423
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope
    Depositor
    Martin Spiess
    Publication
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109423-plasmid-map-sequence-id-260701

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  5. UBH14

    Plasmid
    #28158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    E3 ligase UHRF2 (UHRF2 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    SRA Domain (residues 419-648)
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pcDNA3-sACt-mCherry-NLS

    Plasmid
    #138214
    Purpose
    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.
    Depositor
    Jin Zhang
    Publication
    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sACt-mCherry-NLS (Adcy10 Rat)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138214-plasmid-map-sequence-id-307416

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  8. pcDNA3-sACt-mCherry-NES

    Plasmid
    #138215
    Purpose
    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.
    Depositor
    Jin Zhang
    Publication
    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sACt-mCherry-NES (Adcy10 Rat)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    138215-plasmid-map-sequence-id-307425

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  12. GPKA

    Plasmid
    #61091
    Purpose
    GFP-tagged cAMP-dependent PKA catalytic subunit
    Depositor
    Steven Green
    Publication
    Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pkac2
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61091-plasmid-map-sequence-id-99097

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  15. AW01_pU.CAG.eSpCas9(1.1).rBGpA

    Plasmid
    #199255
    Purpose
    Expression construct encoding the high specificity eSpCas9(1.1) nuclease
    Depositor
    Manuel Goncalves
    Publication
    Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    High specificity eSpCas9(1.1) nuclease
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    K848A K1003A R1060A
    Promoter
    CAG promoter
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199255-plasmid-map-sequence-id-391368

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Showing: 3081 - 3100 of 12310 results