We narrowed to 5,240 results for: codon optimized

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pcDNA3-BaLgp140-8his

Plasmid

#100924

Purpose

Mammalian expression plasmid for soluble gp140 from the BaL HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader sequence and Purification tag after D6…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pVRa20_992

Plasmid

#49673

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF20_992

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA4

Plasmid

#136662

Purpose

Expresses human codon-optimized AcrIIA4 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA4

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMIEG3-BCL6

Plasmid

#40339

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

BCL6 (BCL6 Human)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Mutation

optimized translation initiation codon

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pVRa42_4454

Plasmid

#49703

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF42_4454

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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GB1-ALIX(P801G)

Plasmid

#180025

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

P801G

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1184

Plasmid

#205965

Purpose

NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-miniTurbo-NES-YFP-STOP-L2

Plasmid

#127359

Purpose

gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTMiniTrex-mCherry-Shark2

Plasmid

#233144

Purpose

Serves as a template plasmid for amplifying Shark2 (Tet-ON) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark2

Use

Prokaryotic expression

Tags

3x Ty tag

Mutation

Codon optimized for Trypanosoma cruzi

Available Since

March 20, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pSP64T-hLbCpf1

Plasmid

#107187

Purpose

plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Promoter

sp6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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BPK2797

Plasmid

#72251

Purpose

Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135V, G1218R, R1335Q, and T1337R

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

Plasmid

#129530

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIC3Nme
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAT15547_PEmax-SuperFi-Cas9

Plasmid

#184377

Purpose

Mammalian expression of SuperFi-Cas9 PEmax prime editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

PEmax-SuperFi-Cas9

Use

CRISPR

Tags

SV40 NLS and c-myc NLS

Expression

Mammalian

Mutation

H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT3TS-zAsCpf1

Plasmid

#107185

Purpose

plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

AsCpf1

Use

CRISPR

Tags

NLS-3xHA

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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NylB'-SCY_pET21b

Plasmid

#215417

Purpose

Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB'-SCY

Expression

Bacterial

Mutation

R187S/F264C/D370Y

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-AD8-QES.i08.c04

Plasmid

#123259

Purpose

Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (AD8) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations P124D, …

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

Plasmid

#129532

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIA4
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRGEB-Cas9-VirD2 (PCO)

Plasmid

#139802

Purpose

Expressing Cas9-VirD fusion under ubiquitin promoter

Depositor

Magdy Mahfouz

Article

Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

Insert

Cas9-VirD2

Tags

Cas9-VirD2

Expression

Plant

Promoter

Ubiquitin

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLEXm-GST-ATG14

Plasmid

#99329

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

ATG14 (ATG14 Human)

Tags

GST-TEV tag

Expression

Mammalian

Mutation

Codon optimized

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA2

Plasmid

#136661

Purpose

Expresses human codon-optimized AcrIIA2 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA2

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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