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pYY10-2µ-TP901

Plasmid

#235771

Purpose

2µ plasmid for constitutive expression of TP901 under RNR2 promoter.

Depositor

Chang Liu

Publication

Pisera et al ACS Synth Biol. 2025 Apr 18;14(4):1002-1

Insert

TP901 integrase

Expression

Yeast

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pDIV151

Plasmid

#226180

Purpose

Expresses Cas9 in a backbone compatible with K. phaffii.

Depositor

Uffe Mortensen

Publication

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

Cas9

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

GAP1

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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wg186Fluc

Plasmid

#177436

Purpose

In vitro synthesis of an mRNA encoding Firefly luciferase with A[62] tail.

Depositor

Wendy Gilbert

Publication

Rojas-Duran et al RNA. 2012 Dec;18(12):2299-305. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Firefly luciferase

Use

Luciferase; For in vitro transcription

Tags

None

Promoter

T7

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB8748

Plasmid

#126918

Purpose

Plasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtE

Depositor

Irina Borodina

Publication

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Expression

Yeast

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0532

Plasmid

#133502

Purpose

Expression of mTagBFP2 targeted to the nucleus with one NLS signals

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(tdh1)-NLSSV40-mTagBFP2-terminator(tdh1)-kanMX

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pK3FS-GAL1

Plasmid

#85778

Purpose

N-ICE plasmid pK3FS-GAL1

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pK3FS-CYC1

Plasmid

#85779

Purpose

N-ICE plasmid pK3FS-CYC1

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-CYC1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDZ514 pURA MET25p PP7-PS-2x-yeGFP

Plasmid

#72230

Purpose

Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

Depositor

Daniel Zenklusen

Publication

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Expression

Yeast

Promoter

MET25

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRS306:Met3p-mCherry-Tub1

Plasmid

#37554

Depositor

Wei-Lih Lee

Publication

Markus et al Cytoskeleton (Hoboken). 2011 Mar;68(3):1

Insert

Met3p-mCherry-Tub1

Expression

Bacterial

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-OsTIR1(F74A)

Plasmid

#140731

Purpose

OsTIR1(F74A)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74A)-P2A-mAID-EGFP-NES

Use

AAV

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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SMC2-mAC donor (Hygro)

Plasmid

#140648

Purpose

SMC2 tagging with mAID-Clover

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SMC2 (SMC2 Human)

Use

CRISPR

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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cyterm-ymtq2 pDRF1-GW

Plasmid

#168083

Purpose

Expresses Cyterm-ymTq2 to check for monomericity.

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

cyterm-ymTq2

Expression

Yeast

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mKate2-T2A-mTurquoise2

Plasmid

#118426

Purpose

Produces equimolar expression of mKate2 and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mKate2-T2A-mTq2

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mScarlet-T2A-mTurquoise2

Plasmid

#118431

Purpose

Produces equimolar expression of mScarlet and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mScarlet-T2A-mTurquoise2

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-Purg1-3HA

Plasmid

#39351

Depositor

Jurg Bahler

Publication

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA and kanMX6 module - reverse orientation

Available since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only
pFA6a-TRP1-PGAL1-GFP

Plasmid

#41615

Depositor

John Pringle

Publication

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP(S65T), TRP1 reverse orientation, and yeast AD…

Available since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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p2GB42/Kan

Plasmid

#17260

Depositor

Stuart Schreiber

Publication

Huang et al Proc Natl Acad Sci U S A. 1997 Dec 9. 94

Type

Empty backbone

Tags

B42•HA

Expression

Yeast

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pBM4607

Plasmid

#18795

Depositor

Mark Johnston

Publication

Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

Insert

Sir4 (SIR4 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

aa 951-1200

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pK_P-GAP-ACC1*

Plasmid

#126740

Purpose

yeast plasmid overexpressing ACC1 from Komagataella phaffii

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

acc1*

Expression

Yeast

Mutation

T3452G

Promoter

GAP

Available since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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p406MET25

Plasmid

#17421

Depositor

Nicolas Buchler, Fred Cross

Insert

MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

MET25 promoter is between SacI and XbaI restricti…

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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