We narrowed to 6,115 results for: cat.2

1. pcDNA3-FLAG-mTET2 (N500)

   Plasmid

   #89897

   Purpose

   Expresses mouse TET2 N500 truncation in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N-term 500 truncation of mouse TET2 (contains fir…

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETM33_Nsp3c_SUD-C

   Plasmid

   #156471

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3c_SUD-C (ORF1ab Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-myc-FRNK

   Plasmid

   #74481

   Purpose

   This plasmid was constructed in order to characterize the function of Proline-rich tyrosine kinase 2 (PYK2).

   Depositor

   J Thomas Parsons

   Article

   Xiong et al J Cell Biol. 1997 Oct 20;139(2):529-39.

   Insert

   FRNK (PTK2 Chicken)
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   FRNK is the C-terminal, noncatalytic domain of th…

   Promoter

   CMV

   Available since

   Aug. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pGex-4T-2_LC3A

   Plasmid

   #73946

   Purpose

   For recombinant expression of human LC3A/MAP1LC3A in E. coli

   Depositor

   Dieter Willbold

   Article

   Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

   Insert

   LC3A (MAP1LC3A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pPN458

   Plasmid

   #137876

   Purpose

   PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRa;mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGex-4T-2_GEC1

   Plasmid

   #73945

   Purpose

   For recombinant expression of human GEC1/GABARAPL1 in E. coli

   Depositor

   Dieter Willbold

   Article

   Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

   Insert

   GEC1 (GABARAPL1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pPN459

   Plasmid

   #137875

   Purpose

   PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc-GFP-dSUMOdeltaC

   Plasmid

   #85697

   Purpose

   expression of GFP-SUMO1 delta C in Drosophila cells

   Depositor

   Licio Collavin, Giannino Del Sal

   Article

   Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

   Insert

   SUMO (Sumo Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   non-conjugatable SUMO, lacking the C-terminal gly…

   Promoter

   pAc5

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG

   Plasmid

   #70085

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

   Insert

   mutated human serum transferrin (TF Human)
   

   Use

   Tags

   Hexa His tag and N-terminal signal peptide, 4 aa …

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426…

   Promoter

   SV40

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLPE3

    Plasmid

    #209985

    Purpose

    Contains Level 0* Part: E. coli shuttle part (pMB1_cat) for the construction of Level 2 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    E. coli shuttle part (pMB1_cat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. dpf2_L (OZ531)

    Plasmid

    #27208

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting dpf2 (dpf2 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

12. dpf2_R (OZ532)

    Plasmid

    #27209

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting dpf2 (dpf2 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pSRalpha-MSV-mouse Cdk4wt-Tk-neo

    Plasmid

    #115525

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Matsushime et al Cell. 1992 Oct 16;71(2):323-34.

    Insert

    Mouse Cdk4 (Cdk4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

14. Banshee-ShCit-A

    Plasmid

    #85216

    Purpose

    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

    Depositor

    Alex Minella

    Article

    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

    Insert

    shCit-A
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET His6 MBP TEV LIC cloning vector (2M-T)

    Plasmid

    #29708

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET His6 GST TEV LIC cloning vector (2G-T)

    Plasmid

    #29707

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pScalps_puro_mIL-2Rα RK

    Plasmid

    #59920

    Purpose

    Expresses mouse interleukin-2 receptor alpha chain mutated

    Depositor

    Silvia Monticelli

    Article

    Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

    Insert

    interleukin 2 receptor alpha (Il2ra Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changed sequence of the intracellular (C-terminal…

    Promoter

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pSMT3-FBF2 SS/Y

    Plasmid

    #166973

    Purpose

    Expresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575…

    Promoter

    T7

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. zebrafish similar to gpr151 (or gpcr-2037)_L (OZ535)

    Plasmid

    #27212

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) (LOC565170 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

20. zebrafish similar to gpr151 (or gpcr-2037)_R (OZ536)

    Plasmid

    #27213

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) (LOC565170 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only