We narrowed to 7,548 results for: ski

1. pLV/Guide-mCherry

   Plasmid

   #170543

   Purpose

   Expresses mCherry and contains a cassette for U6 driven sgRNA transcription

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ef1-alpha

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-EF1a-i53

   Plasmid

   #216243

   Purpose

   Express i53 in mammalian cells as a HDR enhancer

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   i53
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-U6-gRNA scaffold-UbC-mCherry-PuroR

   Plasmid

   #232183

   Purpose

   Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, hUbC

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ10871

   Plasmid

   #183962

   Purpose

   U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Inserts

   dCas12a(wildtype)
   poly-crRNA array targeting Sox2-Klf4-Oct4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG and U6

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDF0234 pCMV - huDisCas7-11 dead mutant

   Plasmid

   #172509

   Purpose

   Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   pCMV - huDisCas7-11 dead mutant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGDP1 NDM-1

   Plasmid

   #112883

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   NDM-1 (blaNDM-1 Klebsiella pneumoniae)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGDP3 apmA

   Plasmid

   #112881

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   apmA (apmA Staphylococcus aureus)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGDP4 tet(A)

   Plasmid

   #112887

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   tet(A)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Sequence was codon optimized for E. coli K12 usin…

   Promoter

   Plac

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. rs214-minor

   Plasmid

   #113380

   Purpose

   evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   rs2149943 minor allele
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGDP3 rmtB

    Plasmid

    #112880

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    rmtB (RmtB Escherichia coli)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGDP4 ermC

    Plasmid

    #112884

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    ermC (ermM Staphylococcus epidermis)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGDP3 cat

    Plasmid

    #112888

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    cat (Brevibacillus brevis) (cat Brevibacillus brevis)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGDP3 aph(3'')-Ia

    Plasmid

    #112879

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    aph(3'')-Ia (SGR_RS01140 Streptomyces griseus)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET151-GyrA

    Plasmid

    #216229

    Purpose

    S. aureus USA300 GyrA Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2 GFP-GSK3-MAPK

    Plasmid

    #29689

    Purpose

    GFP reporter protein fused to three GSK3 phosphorylation sites

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    GFP glycogen synthase kinase 3 recognition site primed by MAPK
    

    Use

    Tags

    Flag and Flag-EGFP

    Expression

    Mammalian

    Mutation

    recognition sites only, and E3 ligase site

    Promoter

    SP6

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pphiLOV3-N1

    Plasmid

    #178973

    Purpose

    Expresses phiLOV3 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    phiLOV3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET30a(+)-PE2

    Plasmid

    #180015

    Purpose

    Expressing PE2 protein in E.coli for protein purification

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    PE2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Lac operator-controlled T7 promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-fLuc=tdT

    Plasmid

    #120870

    Purpose

    Piggybac transposon plasmid for live animal optical imaging

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    pcDNA3-fLuc=tdT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Brachyury-eGFP

    Plasmid

    #21063

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Brachyury promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPV-EF1a-EGxxFP-iP-A

    Plasmid

    #204624

    Purpose

    Cloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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