We narrowed to 30,515 results for: Ide

1. EF1A_Hygro_P2A_RUNX1_R64P_Barcode

   Plasmid

   #170927

   Purpose

   Overexpression of RUNX1 R64P mutant protein.

   Depositor

   Prashant Mali

   Article

   Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

   Insert

   RUNX1 (RUNX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   R64P

   Promoter

   Ef1A

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EF1A_Hygro_P2A_RUNX1_T65I_Barcode

   Plasmid

   #170928

   Purpose

   Overexpression of RUNX1 T65I mutant protein.

   Depositor

   Prashant Mali

   Article

   Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

   Insert

   RUNX1 (RUNX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   T65I

   Promoter

   Ef1A

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKT-CNP-inact

   Plasmid

   #211804

   Purpose

   Expresses inactive 2',3'-cyclic nucleotide phosphodiesterase catalytic domain (catalytic residues mutated)

   Depositor

   Emily Weinert

   Article

   Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

   Insert

   2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H73L H153L

   Promoter

   tet

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. B-HeFSpCas9-A695Q

   Plasmid

   #207368

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A695Q
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PET-B-HypaR-SpCas9-NLS-6xHis

   Plasmid

   #207388

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HypaR-SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R661A, N692A, M694A, Q695A, H698A, amino acids 10…

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. B-HeFSpCas9-A926Q

   Plasmid

   #207370

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A926Q
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. B-evoSpCas9-E526K

   Plasmid

   #207365

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-E526K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   M495V, Y515N, R661Q, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. B-evoSpCas9-V495M

   Plasmid

   #207363

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-V495M
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Y515N, K526E, R661Q, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. B-HeFSpCas9-A1060R

   Plasmid

   #207372

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A1060R
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …

   Promoter

   CBh

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. B-HeFSpCas9-A1003K

    Plasmid

    #207371

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A1003K
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …

    Promoter

    CBh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. B-HeFSpCas9-A848K

    Plasmid

    #207369

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A848K
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. B-HeFSpCas9-A661R

    Plasmid

    #207367

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A661R
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. B-evoSpCas9-Q661R

    Plasmid

    #207366

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-Q661R
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    M495V, Y515N, K526E, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. B-evoSpCas9-N515Y

    Plasmid

    #207364

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-N515Y
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    M495V, K526E, R661Q, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PET-B-HeFSpCas9-NLS-6xHis

    Plasmid

    #207392

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

    Promoter

    T7

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p35S_EVDdi

    Plasmid

    #167120

    Purpose

    Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) lacking the intron under CaMV 35S promoter.

    Depositor

    Olivier Voinnet, Arturo Marí-Ordóñez

    Article

    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

    Insert

    EVADE (EVD) (AT5G17125 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Deleted intron from full length element

    Promoter

    CaMV 35S

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETM33_Nsp3c_SUD-C

    Plasmid

    #156471

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3c_SUD-C (ORF1ab Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-X1-Neo-PMP34-DDRGK1-dTM-HA

    Plasmid

    #139862

    Purpose

    Lentiviral expression of PMP34-DDRGK1-dTM-HA

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    PMP34-DDRGK1-dTM (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    recombinant fusion of PMP34 (full length) with DD…

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. B-HypaSpCas9

    Plasmid

    #126763

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-HypaSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N692A; M694A; Q695A; H698A; amino acids 1005-1013…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-FLAG-B-eSpCas9

    Plasmid

    #126772

    Purpose

    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A; K1003A; R1060A; amino acids 1005-1013 repl…

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge