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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,000 results for: pcr

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Showing: 3161 - 3180 of 8000 results

  2. GPR151-Tango

    Plasmid
    #66327
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR151 (GPR151 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66327-plasmid-map-sequence-id-344663

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  4. YFP(1-158)-beta-5 in pcDNAI/Amp

    Plasmid
    #54470
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Insert
    YFP(1-158)/beta-5 (GNB5 Aequorea victoria, Human)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54470-plasmid-map-sequence-id-114865

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  5. GPR84-Tango

    Plasmid
    #66377
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR84 (GPR84 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pDONR P2R-P3-IRES_ECFP

    Plasmid
    #48354
    Purpose
    Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.
    Depositor
    Anna Planas, Tomas Santalucia
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    IRES_ECFP
    Use
    Gateway Cloning
    Mutation
    ECFP translation is mediated by an IRES. Contains…
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48354-plasmid-map-sequence-id-70631

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  8. pATF4-donor

    Plasmid
    #113805
    Purpose
    CRISPR donor plasmid to tag human transcription factor ATF4 with GFP
    Depositor
    Kevin White
    Insert
    ATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
    Use
    CRISPR
    Mutation
    rs140428747
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113805-plasmid-map-sequence-id-231035

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  9. pZC13 (pFA6a-TEV-6xGly-V5-HphMX)

    Plasmid
    #44085
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44085-plasmid-map-sequence-id-61696

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  12. pTF273 (pFA6a-PSc-6xGly-12xHis-HphMX)

    Plasmid
    #44075
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44075-plasmid-map-sequence-id-61677

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  13. pTF269 (pFA6a-PSc-6xGly-12xHis-KanMX)

    Plasmid
    #44087
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44087-plasmid-map-sequence-id-61673

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  16. pHyg-PCUP1-AID*(N)

    Plasmid
    #99529
    Purpose
    N-terminal AID* degron cassette with CUP1 promoter and HygR marker for S. cerevisiae
    Depositor
    Helle Ulrich
    Insert
    IAA17(71-117) (AXR3 Mustard Weed)
    Use
    Pcr template for yeast tagging cassette
    Promoter
    CUP1
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99529-plasmid-map-sequence-id-192013

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  18. pZC13 (pFA6a-TEV-6xGly-V5-HphMX)

    Plasmid
    #44085
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44085-plasmid-map-sequence-id-61696

    Enlarge

  19. GPR84-Tango

    Plasmid
    #66377
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR84 (GPR84 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. MRGPRX1-Tango

    Plasmid
    #66439
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MRGPRX1 (MRGPRX1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 3161 - 3180 of 8000 results