Addgene

We narrowed to 31,294 results for: Eng

Showing: 451 - 480 of 31294 results

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pCfB6637

Plasmid

#212696

Purpose

gRNA targeting to the intergradtion site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB8860

Plasmid

#212697

Purpose

gRNA targeting to the intergradtion site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8856

Plasmid

#212701

Purpose

gRNA targeting to the intergradtion site C3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site C3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8866

Plasmid

#212703

Purpose

gRNA targeting to the intergradtion site F3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site F3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB6630

Plasmid

#212700

Purpose

gRNA targeting to the intergradtion site C3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site C3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB6638

Plasmid

#212698

Purpose

gRNA targeting to the intergradtion site E4

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E4

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS1037

Plasmid

#169616

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1035

Plasmid

#169614

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (PmPGK1-IgkSS-Nluc-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1032

Plasmid

#169612

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::PhCMV-IgkSS-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1031

Plasmid

#169611

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (PhCMV-IgkSS-Nluc-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1033

Plasmid

#169613

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::A2-pA::PhCMV-IgkSS-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS1036

Plasmid

#169615

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::PmPGK1-IgkSS-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-T7Max-UTR1-T500

Plasmid

#178418

Purpose

Empty backbone. T7Max promoter

Depositor

Kate Adamala

Article

Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7Max

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-T7Max-UTR1-NTerminus8xHis-T500

Plasmid

#178421

Purpose

Empty backbone. T7Max promoter with N-His Tag

Depositor

Kate Adamala

Article

Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Tags

8xHis

Expression

Bacterial

Mutation

Promoter

T7Max

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GXB

Plasmid

#160926

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-Aga1p-FAPB2.3.6L1TAG

Plasmid

#158143

Purpose

Yeast display reporter for non-display yeast strains (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

FAPB2.3.6L1TAG

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PHBA03

Plasmid

#83537

Purpose

Constitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genes

Depositor

Claudia Vickers

Article

Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

Insert

pTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t

Use

Tags

Expression

Yeast

Mutation

Promoter

pTEF1

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas73

Plasmid

#82398

Purpose

Δccm:Km, used to delete ccm operon

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

ccm deletion

Use

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pDS3-aurJ

Plasmid

#70152

Purpose

Fusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

aurJ

Use

Tags

Expression

Yeast

Mutation

Promoter

GPD1

Available since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only
pDS2

Plasmid

#70135

Purpose

CEN/ARS yeast shuttle vector with DS2 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

DS2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

AgTEF1

Available since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pBbB0a-RFP

Plasmid

#35371

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbB0k-RFP

Plasmid

#35370

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 7, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbS3c-RFP

Plasmid

#35311

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ProS

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbE3c-RFP

Plasmid

#35305

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ProS

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
J72008 bacterial strain with plasmid

Bacterial Strain

#26187

Depositor

Christopher Anderson

Article

Anderson et al J Biol Eng. 2010 . 4(1):1.

Bacterial Resistance

Ampicillin

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only
pNVLTv2-pvdIJD-Prha-base

Plasmid

#169244

Purpose

Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction)

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

rhaRS

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhaS

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRK793

Plasmid

#8827

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219V mutant

Use

Tags

His, MBP (with TEV), and polyarginine

Expression

Bacterial

Mutation

S219V mutation (improves stability)

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pHB-3

Plasmid

#140956

Purpose

For PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.

Depositor

Kang Zhou

Article

Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

Insert

phaCAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHB-4

Plasmid

#140957

Purpose

For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

Depositor

Kang Zhou

Article

Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

Insert

phaCAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPPC009.106

Plasmid

#171142

Purpose

Expression of J106 scRNA on pBBR1-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J106 scRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge