We narrowed to 1,875 results for: ADAP

1. EC.SurA.pTYB1.a21-281&390-428.M231R

   Plasmid

   #15958

   Depositor

   David McKay

   Article

   Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

   Insert

   Escherichia coli surA delta PPIase domain 2
   

   Use

   Tags

   intein

   Expression

   Bacterial

   Mutation

   residues 282-389 deleted; M231R mutation

   Promoter

   Available since

   Nov. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

2. μ2-HA-D176A

   Plasmid

   #32754

   Depositor

   Alexander Sorkin

   Article

   Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

   Insert

   μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D176A

   Promoter

   CMV

   Available since

   Nov. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pCMVtight-UPRT-T2A-RFP-IRES-CD

   Plasmid

   #126677

   Purpose

   Doxycycline inducible expression of UPRT and CD along with reporter RFP

   Depositor

   Joan Massague

   Article

   Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

   Insert

   UPRT-T2A-RFP-IRES-CD (FCY1 Toxoplasma gondii, Budding Yeast)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   tight TRE

   Available since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-TS2126 RNA Ligase

   Plasmid

   #119941

   Purpose

   Express TS2126 RNA ligase in E. coli

   Depositor

   Takashi Ito

   Article

   Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

   Insert

   TS2126 RNA ligase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-FADD

   Plasmid

   #78802

   Purpose

   To overexpress FADD in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Nat Commun. 2012;3:978. doi: 10.1038/nco

   Insert

   FADD (FADD Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

   Plasmid

   #117687

   Purpose

   U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

   Depositor

   Erik Sontheimer

   Article

   Gao et al Methods Enzymol. 2019;616:365-383. doi:

   Inserts

   Kanamycin cassette
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 myc card9

   Plasmid

   #16253

   Depositor

   William Kaelin

   Article

   Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

   Insert

   card9 (CARD9 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. psigma1-EGFP

   Plasmid

   #53611

   Purpose

   Expresses sigma1 of adaptor protein of AP1

   Depositor

   Tom Kirchhausen

   Article

   Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

   Insert

   sigma 1 (Ap1s1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-AMPKsub-YPet-NES

   Plasmid

   #84635

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   AMPKsub-YPet-NES
   

   Use

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

    Plasmid

    #79614

    Purpose

    Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Inserts

    SpCas9
    PmCDA1
    

    Use

    Tags

    SH3 domain and SHL

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1 and pGal10

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-mCherry-LKB1(KD)

    Plasmid

    #84640

    Purpose

    mCherry-tagged, LKB1(K78I); kinase-dead mutant

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    mCherry-LBK1(K78I) (STK11 Human, Synthetic)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Lysine 78 mutated to Isoleucine; kinase dead

    Promoter

    CMV

    Available since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Intersectin I SH3 A domain (human)

    Plasmid

    #47413

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain A (ITSN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only SH3 domain A

    Promoter

    tac

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pGEX-6P-2_dCrk

    Plasmid

    #131146

    Purpose

    Expresses GST::dCrk (based on crk isoform RA).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79619

    Purpose

    Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    3xFlag-PmCDA1

    Expression

    Mammalian

    Mutation

    D10A for nickase Cas9

    Promoter

    pCMV

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79621

    Purpose

    Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Mammalian

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pCMV

    Available since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Myc-Cdh1

    Plasmid

    #28127

    Depositor

    Pumin Zhang

    Article

    Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

    Insert

    fzr1 (FZR1 Human)
    

    Use

    Mouse Targeting and Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

17. Intersectin I SH3 B domain (human)

    Plasmid

    #47414

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain B (ITSN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only SH3 domain B

    Promoter

    tac

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pMT2-MEKK3-K392A

    Plasmid

    #21585

    Depositor

    John Kyriakis

    Article

    Chadee et al Mol Cell Biol. 2002 Feb . 22(3):737-49.

    Insert

    mitogen-activated protein kinase kinase kinase 3 (MAP3K3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lysine 392 to Arginine

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pCRII-TOPO GFR alpha 1

    Plasmid

    #119898

    Purpose

    Mouse GFR alpha 1 In situ hybridization probe

    Depositor

    David Ginty, Wenqin Luo

    Article

    Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

    Insert

    GDNF family receptor alpha-1 precursor (Gfra1 Mouse)
    

    Use

    In situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Intersectin I SH3 C domain (human)

    Plasmid

    #47415

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain C (ITSN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only SH3 domain C

    Promoter

    tac

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only