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Plasmid#207679PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-sTagRFP cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
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pCMV-PRLSS-yATOM_SH2-KDEL
Plasmid#209714PurposeMammalian expression of y-ATOM biosensor for SH2 localized to endoplasmic reticulumDepositorInsertPRLSS-yATOM_SH2-KDEL
UseTagsPRLSSExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MBP-yATOM_WDR5 (R80A)
Plasmid#209713PurposeMammalian expression of y-ATOM biosensor for WDR5 with binding null R80A mutationDepositorInsertMBP-yATOM_WDR5 (R80A)
UseTagsMBPExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PRLSS-NbGFP_miRFP670nano3-KDEL
Plasmid#209718PurposeMammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulumDepositorInsertPRLSS-NbGFP_miRFP670nano3-KDEL
UseTagsPRLSSExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PRLSS-Clover-KDEL
Plasmid#209720PurposeMammalian expression of clover localized to endoplasmic reticulumDepositorInsertPRLSS-Clover-KDEL
UseTagsPRLSSExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cox4-yATOM_SH2
Plasmid#209715PurposeMammalian expression of y-ATOM biosensor for SH2 localized to mitochondriaDepositorInsertCox4-yATOM_SH2
UseTagsCos4ExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-4 β4
Plasmid#198327PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 β4 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-4 β4
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationContains L480S substitutionPromoterADH1Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-2 σ2
Plasmid#198345PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-2 σ2
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationR42G substitution, and silent substitutions in co…PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1C
Plasmid#198336PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1C fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1C
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationS148C substitutionPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-AP-3 σ3A
Plasmid#198347PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 σ3A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 σ3A
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-AP-3 σ3B
Plasmid#198348PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 σ3B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 σ3B
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-tepsin
Plasmid#196728PurposeExpresses tepsin-GFP in mammalian cellsDepositorInserttepsin (TEPSIN Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-iGFP-DRS2
Plasmid#177104PurposeExpresses iGFP-Drs2 in yeast cellsDepositorInsertDRS2 (DRS2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-AUR1-msGFP2
Plasmid#177101PurposeExpresses Aur1-msGFP2 in yeast cellsDepositorInsertAUR1 (AUR1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RBD2-6xmGFP
Plasmid#177109PurposeExpresses Rbd2-6xmEGFP in yeast cellsDepositorInsertRBD2 (RBD2 Budding Yeast)
UseTags6xmEGFPExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-SEC2-msGFPx3
Plasmid#177110PurposeExpresses Sec2-msGFPx3 in yeast cellsDepositorInsertSEC2 (SEC2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huMb3Cas12a
Plasmid#174677PurposeExpresses 6xHis-MBP tagged Mb3Cas12aDepositorArticleInserthuMb3Cas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huCMaCas12a
Plasmid#174679PurposeExpresses 6xHis-MBP tagged CMaCas12aDepositorArticleInserthuCMaCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huMb2Cas12a
Plasmid#174676PurposeExpresses 6xHis-MBP tagged Mb2Cas12aDepositorArticleInserthuMb2Cas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huPxCas12a
Plasmid#174675PurposeExpresses 6xHis-MBP tagged PxCas12aDepositorArticleInserthuPxCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only