Addgene

We narrowed to 21,975 results for: Sis

Showing: 3181 - 3200 of 21975 results

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GB4666

Plasmid

#215271

Purpose

pUPD2 for the Arabidopsis thaliana ABA-responsive MAPKKK18 promoter.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

promMAPKKK18

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pNK875

Plasmid

#219735

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtTCTP

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter from Arabidopsis translationally controlled tumor protein (AtTCTP) gene

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

AtTCTP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDSK519-cspB

Plasmid

#207162

Purpose

Expresses codon-optimized cspB from Clavibacter michiganensis in a broad host range vector

Depositor

Gitta Coaker

Article

Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

Insert

cspB

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

nptII

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA329

Plasmid

#214749

Purpose

High copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RBS(22821)-mcherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA327

Plasmid

#214747

Purpose

Low copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RBS(22821)-mcherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA325

Plasmid

#214742

Purpose

Medium copy glmS-nadE plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA324

Plasmid

#214741

Purpose

Low copy glmS-nadE plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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Noodles Plasmid

Plasmid

#212613

Purpose

An all-in-one system for on-target efficiency analysis of CRISPR guide RNAs

Depositor

Ilya Vinnikov

Article

Lin et al MethodsX. 2023 Nov 15;12:102481. doi: 10

Type

Empty backbone

Use

CRISPR and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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MP6-SlugABEN-738

Plasmid

#214348

Purpose

Expresses N-terminus of ABE, and untargeted Bacterial Mutagenesis

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

dnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA326

Plasmid

#214743

Purpose

High copy glmS-nadE plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSV014

Plasmid

#214740

Purpose

High copy thyA-infA plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSV013

Plasmid

#214739

Purpose

Medium copy thyA-infA plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSV015

Plasmid

#214738

Purpose

Low copy thyA-infA plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pEG302-JMJ14-ZF

Plasmid

#200913

Purpose

Express JMJ14-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Article

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

JMJ14

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302-HD2A-ZF

Plasmid

#200915

Purpose

Express HD2A-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Article

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

HD2A

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NKX6-2-Venus S246A

Plasmid

#203715

Purpose

human NKX6-2 S246A phosphoresistant mutant

Depositor

Federico Herrera

Article

Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

Insert

NKX6-2 (S246A) fused with Venus (NKX6-2 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

NKX6-2 (S246A)

Promoter

CMV

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NKX6-2-Venus T252A

Plasmid

#203717

Purpose

human NKX6-2 T252A phosphoresistant mutant

Depositor

Federico Herrera

Article

Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

Insert

NKX6-2 (T252A) fused with Venus (NKX6-2 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

NKX6-2 (S252A)

Promoter

CMV

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB560-NADK2

Plasmid

#203760

Purpose

For Agrobacterium transformation. tagRFP fused Arabidopsis thaliana NAD KINASE2 (NADK2) under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

NAD KINASE2

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE1176

Plasmid

#202774

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE1177

Plasmid

#202775

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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