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BS.YxiN.Twin1.a1-368.wt

Plasmid

#13528

Depositor

David McKay

Article

Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

Insert

B. subtilis DEAD-box helicase YxiN first two domains

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

deleted amino acid residues 369-479

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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piHGpd/T22I

Plasmid

#1110

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with threonine 22 changed to isoleucine; re…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/G81S

Plasmid

#1111

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glycine 81 changed to serine; regulate…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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piHGpd/G313S

Plasmid

#1114

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glycine 313 changed to serine; regulat…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/E381K

Plasmid

#1115

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glutamic acid 381 changed to lysine; r…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/A587T

Plasmid

#1116

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with alanine 587 changed to threonine; regu…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/A41V

Plasmid

#1117

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with alanine 41 changed to valine; regulate…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
LentiV_Neo

Plasmid

#108101

Purpose

Lentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector)

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

FLAG tag

Expression

Mammalian

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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PB-TRE-EGFP-EF1a-rtTA

Plasmid

#104454

Purpose

PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

Depositor

Volker Busskamp

Article

Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

Inserts

enhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3

Use

Piggybac

Expression

Mammalian

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GINKO1

Plasmid

#113112

Purpose

Mammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

GINKO1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pJM253 (miniCTX1-rhaSR-PrhaBAD)

Plasmid

#110571

Purpose

Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Expression

Worm

Promoter

rab-3

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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ER-RpHLuorin2

Plasmid

#171717

Purpose

Luminal ER expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 targeted to ER

Tags

KDEL ER-retention signal and Signal sequence of h…

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HDAC2

Plasmid

#222553

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HDAC2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HDAC2 (HDAC2 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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G-CaMP3

Plasmid

#22692

Purpose

a single-wavelength GCaMP2-based genetically encoded calcium indicator

Depositor

Loren Looger

Article

Tian et al Nat Methods. 2009 Dec;6(12):875-81.

Insert

G-CaMP3

Expression

Mammalian

Mutation

N363D mutation (increased fluorescence)

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pC1-HyPerRed-mito

Plasmid

#60247

Purpose

Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HyPerRed-mito

Tags

Mitochondrial targeting signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pTet-IRES-EGFP

Plasmid

#64238

Purpose

Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

Depositor

Maria Lung

Article

Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

pTet

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pLifeAct_mScarlet-i_N1

Plasmid

#85056

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-TRBP

Plasmid

#50351

Purpose

bacterial expression of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

Insert

TRBP (TARBP2 Human)

Tags

His and Myc

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pmScarlet_peroxisome_C1

Plasmid

#85063

Purpose

In vivo visualization of the peroxisome (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Peroxisomal targeting sequence

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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