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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,618 results for: cre encoding plasmid

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Showing: 3221 - 3240 of 5618 results

  5. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid
    #224698
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224698-plasmid-map-sequence-id-444264

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  7. SMT000

    Plasmid
    #216849
    Purpose
    Plasmid that encodes a sfGFP gene under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. Chloramphenicol resistant.
    Depositor
    Polly Fordyce
    Article
    Thompson et al Adv Mater Interfaces. 2025 Feb 3;12(3):2
    Insert
    Superfolder Green Fluorescent Protein
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    S30R, Y39N, N105T, Y145F, I171V, and A206V
    Promoter
    T7
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    216849-plasmid-map-sequence-id-433256

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  8. pcDNA5-FRT-TO-Nsp13-HF_GC3opt

    Plasmid
    #157716
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157716-plasmid-map-sequence-id-307912

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  9. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

    Plasmid
    #157699
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157699-plasmid-map-sequence-id-307863

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  20. pBAD-MTX-u36_Snifit

    Plasmid
    #119045
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u36_Snifit
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    36TAG R44L H45Q
    Promoter
    pBAD
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119045-plasmid-map-sequence-id-229505

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Showing: 3221 - 3240 of 5618 results