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pJMC-7T-Empty

Plasmid

#197857

Purpose

Level 1 module 7T empty

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT Linker

Plasmid

#192307

Purpose

Gateway entry vector for cloning

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

Linker

Use

Gateway Cloning

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCJ164

Plasmid

#175057

Purpose

rbcLXS7002 integrative plasmid expresses DRE recombinase as a translational fusion to rbcL

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

DRE

Use

Unspecified

Promoter

rbcLXS

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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GtCCR1_EYFP_pcDNA3.1

Plasmid

#159120

Purpose

This plasmid encodes GtCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

Insert

GtCCR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRS315Nab3Q->E

Plasmid

#110538

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

O'Rourke et al PLoS One. 2016 Mar 8;11(3):e0150865. doi

Insert

NAB3 (NAB3 Budding Yeast)

Expression

Yeast

Mutation

Q to E mutations in Nab3 low complexity domain

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pPN10-gDM1d

Plasmid

#114387

Purpose

pPN10 with gRNA for spCRISPR-Cas9 against the 3' UTR of the DMPK gene target: TGCGAACCAACGATAGGTG PAM: GGG

Depositor

Vincent Dion

Article

Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

Insert

gDM1d

Use

CRISPR

Availability

Academic Institutions and Nonprofits only

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pJSK659

Plasmid

#205128

Purpose

Expression of GFP-SnxA in mammalian cells

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBSDONR P1-P4

Plasmid

#71744

Purpose

ampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloning

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Type

Empty backbone

Use

Gateway entry vector for use in multisite gateway…

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMC-6-ccdB-9

Plasmid

#197812

Purpose

Level 0 J6-J9 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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p-cyclinB1-mcherry (1958)

Plasmid

#26063

Depositor

Jonathon Pines

Article

Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

Insert

Cyclin B1 (CCNB1 Human)

Tags

mcherry

Expression

Mammalian

Available Since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pJV0047

Plasmid

#205139

Purpose

Expression of 6xHis-EGFP-2xPX in bacteria

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

tandem 2xPX domain of SnxA

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pSAM_Ec

Plasmid

#102939

Purpose

Transposon mutagenesis and Tn-seq

Depositor

Matthew Mulvey

Article

Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

Insert

mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase

Expression

Bacterial

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEM790

Plasmid

#242607

Purpose

T-DNA plasmid, Level 2 destination vector, replaces pMIN-VS1

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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DS-SPcas

Plasmid

#48645

Purpose

Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
tracrRNA precursor

Use

CRISPR

Promoter

proC and tracrRNA promoter

Available Since

Dec. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pT7-CalfluxVTN

Plasmid

#83926

Purpose

Bioluminescent Reporter of Intracellular Calcium

Depositor

Carl Johnson

Article

Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

Insert

CalfluxVTN

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLX302 Luciferase-V5 puro

Plasmid

#47553

Purpose

Lentiviral expression of V5-tagged Luciferase in mammalian cells

Depositor

Kevin Janes

Article

Kang et al Sci Signal. 2013 Aug 6;6(287):rs13. doi:

Insert

Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

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sox10:mCherry

Plasmid

#98695

Purpose

I-SceI meganuclease-mediated mCherry transgenesis in zebrafish

Depositor

Thomas Look

Article

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

mCherry

Use

Zebrafish expression

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_67_pKD46.10

Plasmid

#178942

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available Since

March 1, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSLIK DNM1L-V5 neo

Plasmid

#220363

Purpose

Lentiviral expression vector for an inducible V5-tagged DNM1L construct

Depositor

Kevin Janes

Insert

DNM1L (DNM1L Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJV0042

Plasmid

#205138

Purpose

Expression of 6xHis-EGFP-SnxA in bacteria

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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