We narrowed to 8,560 results for: Dos
-
Plasmid#128355PurposegRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNoureothricin (NAT)
UseUnspecifiedPromoterACT1Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-CyrCR1.miR16
Plasmid#128747PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.DepositorInsertCyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
ExpressionMammalianPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC114
Plasmid#118904Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase siteDepositorInsertNcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with TEV protease, two glycinesExpressionInsectPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B
Plasmid#108883PurposeMultisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation)DepositorInsertH2B (human amino acid sequence but different codon usage) (H2BC21 Synthetic, Human)
UseGateway CloningMutationhuman amino acid sequence but different codon usa…Available sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK015
Plasmid#105347PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionMutationBsaI restriction sites removedAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG387
Plasmid#105338PurposeSynthetic biology; gateway destination vector assemblyDepositorTypeEmpty backboneUseSynthetic BiologyAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro.puro-shMePCE_shLARP7
Plasmid#113538PurposeRetroviral vector designed to knock down MePCE and LARP7 simultaneously.DepositorInsertshMePCE and shLARP7 (MEPCE Human)
UseRetroviralAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDM1080
Plasmid#108980PurposeKnockout cassette NourseothricinDepositorInsertKnockout cassette Nourseothricin
UseCre/Lox; Dictyostelium discoideum knockout vectorPromotercoaAAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDM1020
Plasmid#108977Purposeshuttle vector, C-terminal mCherry taggingDepositorTypeEmpty backboneUseDictyostelium discoideum shuttle vectorTagsmCherryAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-hNgAgo
Plasmid#104384Purposehuman codon optimized NgAgo; pCDNA3 vector backbone, mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthNgAgo
Tags3XFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
H14-MBP-SUMO-Cys-Linker-BAF_L58R
Plasmid#101780PurposeExpresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)DepositorInsertBAF (BANF1) (BANF1 Human)
TagsHis14-MBP-SUMOExpressionBacterialMutationL58R mutationPromoterT5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
H14-MBP-SUMO-FLAG-LEM
Plasmid#101778PurposeExpresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)DepositorInsertMAN1 (LEMD3 Human)
TagsHis14-MBP-SUMO-FLAGExpressionBacterialMutationamino acids 6-51 of human MAN1 (LEM domain)PromoterT5Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP
Plasmid#69050Purposelentiviral expression of IKZF3 with Q147H mutation and FLAG tagDepositorInsertIKZF3 (IKZF3 Human)
UseLentiviralTagsFLAG and IRES-GFPExpressionMammalianMutationQ147HPromoterCAGAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
36INS-J23100_AB
Plasmid#78666PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_J23100. [A:36INS-J23100:B]DepositorInsertInsulated J23100 Promoter
UseSynthetic BiologyAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
MO70-hCRACR2A-isoform-c
Plasmid#79591PurposeTransient and retroviral expressionDepositorAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
p123shy1-3HA
Plasmid#67876Purposeencodes shy1 with a C-terminal 3xHA-tag under control of its endogenous promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertshy1
UseExpression in ustilago maydisTagsHAPromotershy1Available sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail
Plasmid#60392PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoterDepositorInsertTUB1 (TUB1 Synthetic, Budding Yeast)
ExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mY
Plasmid#60988PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound citrineDepositorInsertcitrine
UseZebrafishTagsmembrane tagPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mO
Plasmid#60989PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKODepositorInsertmKO
UseZebrafishTagsmembrane tagPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only