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pSLIK-Neo-TGmiR-Gb2

Plasmid

#25746

Purpose

Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 (Gnb2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL3-9465

Plasmid

#115464

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL3-9465 (MBNL3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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FRT2-H2B-EGFP-V5-2A (IG157)

Plasmid

#99623

Purpose

To clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

H2B-EGFP-V5

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRC2_p53DD

Plasmid

#136520

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

p53DD (Trp53 Mouse)

Use

Gateway Cloning

Tags

3x FLAG

Mutation

This construct was created by PCR from the pBabe …

Available Since

Oct. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-PGK-Puro

Plasmid

#110859

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Fascin-Promoter 402 mutation

Plasmid

#89825

Purpose

Fascin promoter for luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Mutation

-370 Smad binding element mutated from CAGAC to T…

Promoter

SV40

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-QKI-6 [N182/17.1R]

Plasmid

#114561

Purpose

Mammalian Expression Plasmid of anti-QKI-6 (Human). Derived from hybridoma N182/17.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-QKI-6 (Human) recombinant mouse monoclonal antibody (QKI Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus-TmiR-G12

Plasmid

#25740

Purpose

Lentiviral vector with Tet-based inducible expression of mouse G alpha 12 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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FRT3-HA-H2B-Cerulean-2A (IG158))

Plasmid

#99624

Purpose

To clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HA-H2B-Cerulean

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-P2A-Puro

Plasmid

#110852

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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NOP FRET sensor

Plasmid

#231809

Purpose

FRET-based intramolecular conformation sensor for human nociceptin receptor NOP

Depositor

Moritz Bünemann

Article

Kirchhofer et al Commun Biol. 2025 Feb 8;8(1):198. doi: 1

Insert

Nociceptin Receptor (PNOC Human)

Tags

mTurq2 and sYFP2 (3rd loop)

Expression

Mammalian

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-ITGB1-V1

Plasmid

#215454

Purpose

Expression vector with integrin beta1 tagged with the BiFC V1 fragment

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

CMV

Available Since

Nov. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-Fbxw7opt

Plasmid

#241795

Purpose

Expresses Fbxw7 gene in mammalian cells.

Depositor

Diego Castrillon

Article

Cuevas et al Proc Natl Acad Sci U S A. 2019 Nov 26. p

Insert

Fbxw7 (FBXW7 Human)

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-PTPN11(D425A,C459S)-Clover

Plasmid

#215520

Purpose

Gateway entry vector with a clover-tagged phosphatase-inactive Shp2 mutant (D425A,C459S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Use

Gateway Cloning

Tags

Clover

Mutation

D425A, C459S (Phosphatase inactive)

Promoter

none

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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GST-GRP78-C73 (pGEX4T1)

Plasmid

#238428

Purpose

Expresses GRP78 with the only the last 73 amino acids at the C terminus fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Delta C17 (pGEX4T1)

Plasmid

#238426

Purpose

Expresses GRP78 with the last 17 amino acids at the C terminus deleted fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Delta C11(pGEX4T1)

Plasmid

#238425

Purpose

Expresses GRP78 with the last 11 amino acids at the C terminus deleted fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Delta C73 (pGEX4T1)

Plasmid

#238427

Purpose

Expresses GRP78 with the last 73 amino acids at the C terminus deleted fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Delta KDEL (pGEX4T1)

Plasmid

#238424

Purpose

Expresses GRP78 Delta KDEL fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-PTPN11(D425A,C459S)

Plasmid

#215515

Purpose

Gateway entry vector with a phosphatase-inactive Shp2 mutant (D425A,C459S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Use

Gateway Cloning

Mutation

D425A, C459S (Phosphatase inactive)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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