We narrowed to 5,068 results for: cre encoding plasmid

1. pESRRG.1.0-gDNA

   Plasmid

   #132473

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   ESRRG (ESRRG Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGABPB2.1.0-gDNA

   Plasmid

   #132457

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   GABPB2 (GABPB2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p35S::HY5

   Plasmid

   #135233

   Purpose

   constitutive expression of HY5 from A. thaliana (AT5G11260; downregulates UPR signaling)

   Depositor

   Armin Djamei

   Article

   Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

   Insert

   HY5 (HY5 Mustard Weed)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Direct-seq_lentiGuide-Puro-Loop2-8A8G

   Plasmid

   #157983

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (Loop2-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Direct-seq_lentiGuide-Puro-Tail-8A8G

   Plasmid

   #157981

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (Tail-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6.3delta4 GPR3-V5

   Plasmid

   #172601

   Purpose

   Lentiviral plasmid expressing c-terminal V5-tagged GPR3

   Depositor

   Robert Screaton

   Article

   Iorio et al Commun Biol. 2021 Jul 23;4(1):907. doi:

   Insert

   G protein-Coupled Receptor 3 (GPR3 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGal-BE3

   Plasmid

   #172409

   Purpose

   Yeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoter

   Depositor

   Leonid Kruglyak

   Article

   Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

   Insert

   BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GALL

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. OTUB1/pRetroX-IRES-DsRed#10

   Plasmid

   #180279

   Purpose

   Plasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).

   Depositor

   Andre Catic

   Article

   Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

   Insert

   human Otubain-1 (OTUB1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-dual-scafffold-2.0 (pBP49)

   Plasmid

   #218931

   Purpose

   The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Insert

   dual scaffolds for CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

    Plasmid

    #157986

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

    Plasmid

    #157984

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G

    Plasmid

    #157985

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. B-HiFi SpCas9

    Plasmid

    #207362

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HiFi SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R691A, amino acids 1005-1013 replaced with two gl…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G

    Plasmid

    #157982

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (Tetra-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG

    Plasmid

    #222852

    Purpose

    Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Use

    Tags

    IgG1_Fc

    Expression

    Mammalian

    Mutation

    L234A, L235A, P329G

    Promoter

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG

    Plasmid

    #222854

    Purpose

    Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Use

    Tags

    IgG1_Fc

    Expression

    Mammalian

    Mutation

    L234A, L235A, P329G

    Promoter

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. B-SpCas9-HF1

    Plasmid

    #126762

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A; R661A; Q695A; Q926A; amino acids 1005-1013…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM6-G6-vioABE

    Plasmid

    #73438

    Purpose

    Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PG6 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. B-Sniper SpCas9

    Plasmid

    #207361

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Sniper SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. eSpCas9-plus

    Plasmid

    #126767

    Purpose

    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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