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pSOX17-T2A-dTom-PGK-Neo

Plasmid

#223190

Purpose

Tomato reporter plasmid targeting the human SOX17 gene

Depositor

Matthew Birket

Article

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

SOX17-T2A-dTomato-PGK-Neo-SOX17 (SOX17 Human)

Use

Cre/Lox; Human targeting

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-FLAG-HAND1-T2A-mTagBFP2

Plasmid

#223192

Purpose

Entry vector containing FLAG-HAND1 with a T2A-mTagBFP2 reporter (attL flanked)

Depositor

Matthew Birket

Article

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

HAND1 (HAND1 Human)

Use

Promoterless entry vector for gateway cloning

Tags

FLAG and T2A-mTagBFP2

Expression

Bacterial

Promoter

None

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pColdI-DDX3_helicase_core_Q360L

Plasmid

#233619

Purpose

Expression of His-tagged helicase core region of DDX3X with Q360L mutation in E.coli

Depositor

Shintaro Iwasaki

Article

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

DDX3X helicase core Gln360Leu (DDX3X Human)

Tags

His6

Expression

Bacterial

Mutation

Gln360Leu

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pColdI-DDX3_helicase_core_Q360P

Plasmid

#233620

Purpose

Expression of His-tagged helicase core region of DDX3X with Q360P mutation in E.coli

Depositor

Shintaro Iwasaki

Article

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

DDX3X helicase core Gln360Pro (DDX3X Human)

Tags

His6

Expression

Bacterial

Mutation

Gln360Pro

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_A8_His

Plasmid

#201718

Purpose

Expression of nanobody

Depositor

Ray Owens

Article

Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

Insert

VHH_A8 (S lama glama (llama))

Tags

Hexahistidine

Expression

Bacterial

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_A10-5_His

Plasmid

#201720

Purpose

Expression of nanobody

Depositor

Ray Owens

Article

Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

Insert

VHH_A10-5 (S lama glama (llama))

Tags

Hexahistidine

Expression

Bacterial

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_B5-5_His

Plasmid

#201721

Purpose

Expression of nanobody

Depositor

Ray Owens

Article

Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

Insert

VHH B5-5 (S lama glama (llama))

Tags

Hexahistidine

Expression

Bacterial

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro 2xStrep-HPC4-Gb5

Plasmid

#218177

Purpose

Used to make a lenti virus for stable expression of the G protein beta 5 subunit in mammalian cells

Depositor

Barry Willardson

Article

Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

Insert

G beta subunit 5 (GNB5 Human)

Use

Lentiviral

Tags

2x Strep tag and HPC4

Expression

Mammalian

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-MXRA5(Trunc2)-COMP5AP-AviTag-9xHis

Plasmid

#157598

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

MXRA5 (MXRA5 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NFASC-COMP5AP-AviTag-9xHis

Plasmid

#157599

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NFASC (NFASC Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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