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Beta1 N391* N562* ectodomain

Plasmid

#221400

Purpose

Mammalian expression of human integrin beta1 N391* N562* ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* ectodomain (ITGB1 Human)

Tags

AVI tag, HRV3C cleavage site, basic coil, HA tag,…

Expression

Mammalian

Mutation

codon optimized for human mature _1 residues Q1 t…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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PMP34-mApple-NFAST(low)

Plasmid

#214418

Purpose

Peroxisome localization of low-affinity NFAST fused to PMP34-Apple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N (SLC25A17 )

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-Prf1-ires-mCherry

Plasmid

#209073

Purpose

Targeting vector for the mouse Prf1 locus to correct prf1 gene

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

Prf1-ires-mCherry HDR template (parts of Prf1 with mutated PAM) (Prf1 Mouse)

Use

CRISPR

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-Prf1-mock-ires-mCherry

Plasmid

#209074

Purpose

Targeting vector for the mouse Prf1 locus to correct prf1 gene

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

Prf1-mock-ires-mCherry HDR template (parts of Prf1(S399Stop) with mutated PAM) (Prf1 Mouse)

Use

CRISPR

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-K142R

Plasmid

#213423

Purpose

Expresses human SKP1 K142R mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-K142R (SKP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-K163R

Plasmid

#213424

Purpose

Expresses human SKP1 K163R mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-K163R (SKP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-Δ220-234-Flag

Plasmid

#210341

Purpose

Expresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

Δ220-234

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-H120N/F121L-Flag

Plasmid

#210340

Purpose

Expresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

H120N/F121L

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-mCherry

Plasmid

#210319

Purpose

Expresses SARS-CoV-2 NSP4 fused with mCherry in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

mCherry

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Hgs-g1)-PGKpuroBFP-W

Plasmid

#105032

Purpose

Lentiviral gRNA plasmid targeting mouse Hgs , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

gRNA targeting Hgs (Hgs Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Rescue myc-PCDHGC5 mRNA

Plasmid

#122229

Purpose

Rescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 (Pcdhgc5 Rat)

Tags

9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…

Expression

Mammalian

Mutation

Silent mutations are in five consecutive codons …

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP-PRM-3R

Plasmid

#112156

Purpose

Mammalian eGFP expression plasmid for PRM-3R.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

PRM-3R (ABL1 Human)

Expression

Mammalian

Mutation

contains only the PRM domain of ABl1

Promoter

CMV

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pHDT2:GUS

Plasmid

#108444

Purpose

Arabidopsis transformation, expresses HDT2 promoter/GUS fusion to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT2 promoter (HD2B Mustard Weed)

Tags

GUS

Expression

Plant

Promoter

HDT2

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHDT2:HDT2-GFP

Plasmid

#108566

Purpose

Arabidopsis transformation, expresses HDT2/GFP fusion with HDT2 promoter to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT2 (HD2B Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

HDT2

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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hs-leonardo

Plasmid

#42065

Depositor

Norbert Perrimon

Article

Li et al Development. 1997 Oct;124(20):4163-71.

Insert

leonardo (14-3-3zeta Fly)

Expression

Insect

Promoter

Hsp70

Available Since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
pRubiG-T2A-cre

Plasmid

#66693

Purpose

Recombine floxed alleles in newborn neurons

Depositor

Bryan Luikart

Article

Williams et al J Neurosci. 2015 Jan 21;35(3):943-59. do

Insert

GFP-T2A-cre

Use

Retroviral

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only
LAMP1-GFP

Plasmid

#16290

Depositor

Ron Vale

Article

Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

Insert

LAMP1/RE72002

Tags

EGFP

Expression

Insect

Promoter

Ac5

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pET15-UbE2D2

Plasmid

#15783

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D2 (UBE2D2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pTW334e

Plasmid

#237315

Purpose

expresses A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Insert

A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAC1801_pmax-dCasRx

Plasmid

#118634

Purpose

Transient transfection; Expresses dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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