We narrowed to 10,039 results for: pET

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LM670

Plasmid

#191552

Purpose

LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

Depositor

David Baker

Publication

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Tags

HIS-tag

Expression

Bacterial

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCIneo-hCHD7-Kozak ATG 3' 3x Flag-bGH polyA

Plasmid

#89459

Purpose

constitutive expression of human CHD7 in mammalian cells

Depositor

Peter Scacheri

Insert

CHD7 (CHD7 Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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His-PIMT

Plasmid

#34852

Depositor

Bruce Downie

Publication

Dinkins et al Plant J. 2008 Jul;55(1):1-13.

Insert

PIMT (PCMT1 Human)

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pAG87 FAST

Plasmid

#130719

Purpose

Expresses His-tagged FAST (also called YFAST) in bacteria

Depositor

Arnaud Gautier

Publication

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

FAST

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-mTagBFP2-TEV-Strep-His

Plasmid

#210824

Purpose

Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

Depositor

Luke Chao

Publication

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

mTagBFP2

Tags

TwinStrep, 10xHis

Expression

Bacterial

Promoter

T7

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-41-4GS-LaG-2

Plasmid

#172766

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-41-4GS-LaG-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

Plasmid

#167268

Purpose

Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

Depositor

Kai Johnsson

Publication

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HOB

Tags

His x10

Expression

Bacterial

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pF711-pET28a-Hs-delta25-MCAD-NHis wt

Plasmid

#85112

Purpose

expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Publication

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta25) MCAD (ACADM Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-GGG-ELP(40nm)-Cys

Plasmid

#91571

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Publication

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(40nm)-Cys

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-HIS-Xyl-Xmod-DocIII

Plasmid

#60865

Purpose

Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C

Depositor

Hermann Gaub

Publication

Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

Inserts

Xyl
Xmod-DocIII

Tags

6xHIS

Expression

Bacterial

Mutation

Threonine 120 to Cysteine in Xylanase domain

Promoter

T7

Available since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pMU131

Plasmid

#66811

Purpose

Shuttle vector with gram-positive origin of replication isolated from a native plasmid of Thermoanaerobacterium saccharolyticum B6A-RI

Depositor

Lee Lynd

Publication

Shaw et al Appl Environ Microbiol. 2010 Jul;76(14):

Type

Empty backbone

Expression

Bacterial

Available since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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S96 ELP

Plasmid

#68393

Purpose

Elastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residue

Depositor

Andrew MacKay

Publication

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

S96 ELP

Expression

Bacterial

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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T7-RelA(KR)

Plasmid

#23251

Depositor

Warner Greene

Publication

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

K218/221R, K310R

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRL128

Plasmid

#40180

Depositor

Eric Cascales

Publication

Gueguen et al Appl Environ Microbiol. 2013 Jan;79(1):3

Inserts

Ptac-lacO
araC-pBAD

Promoter

BAD and tac

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

Plasmid

#183410

Purpose

piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

Depositor

Azim Surani

Publication

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

KRAB-dCas9-ecDHFR

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE3G

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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human p53-(82-360)

Plasmid

#24868

Depositor

Cheryl Arrowsmith

Publication

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 82-360

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
560HTR

Plasmid

#24444

Depositor

Ron Vale

Publication

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis

Expression

Bacterial

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLVX_DSB_Spectrum-V2

Plasmid

#192475

Purpose

Lentiviral DSB Spectrum V2 Reporter System

Depositor

Michael Yaffe

Publication

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, incompleteGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

Plasmid

#213024

Purpose

Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

Depositor

Martin S. Taylor, Oliver Weichenrieder

Publication

Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

Insert

LINE-1 ORF2p (238-1061)

Tags

His6-MBP-3C

Expression

Bacterial

Promoter

T7 / Lac

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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mEmerald-Claudin7-C-12

Plasmid

#54041

Purpose

Localization: Tight Junctions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Claudin7 (CLDN7 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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