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KK501: pMVP (L3-L2) FKBP DD + polyA

Plasmid

#121789

Purpose

pMVP L3-L2 entry plasmid, contains FKBP DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

FKBP Degron Domain + polyA

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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PCTAIRE-1-DN-HA

Plasmid

#1998

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

PCTAIRE-1 (CDK16 Human)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative, D304N in conserved KLAD*FGLAR …

Available Since

Jan. 24, 2006

Availability

Academic Institutions and Nonprofits only
pICSL80036

Plasmid

#68259

Purpose

Level 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Coding sequence, hygromycin phophotransferase II (Escherichia coli)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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HOXB13 (human) MSCV

Plasmid

#8544

Depositor

Corey Largman

Article

Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Insert

HOXB13 (HOXB13 Human)

Use

Retroviral

Expression

Mammalian

Mutation

contains 141 NT of 5' UTR prior to ATG start

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pBluescriptKS(-)-FRBx2

Plasmid

#104476

Purpose

Plasmid carrying FRBx2 with C-terminal HA tag.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

FRBx2 (MTOR Human)

Use

Phagemid

Tags

HA tag

Mutation

includes only the FRB domain

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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KTK_251

Plasmid

#180546

Purpose

Entry vector containing mScarlet

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

mScarlet

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-SpCas9-HF4

Plasmid

#108295

Purpose

pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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LC501: pMVP (L3-L2) mCherry + polyA

Plasmid

#121765

Purpose

pMVP L3-L2 entry plasmid, contains mCherry + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry + polyA

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Cdk6-DN

Plasmid

#1867

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pCASP-SptP167-3G124-HilA

Plasmid

#153322

Purpose

Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP167-3G124 DARPin

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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sg2.02 MUC4.1

Plasmid

#101152

Purpose

gRNA with two MCP binding sites

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Promoter

mU6

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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IZ401: pMVP/Lenti/Neo-DEST

Plasmid

#121850

Purpose

pMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-TD-HilA

Plasmid

#153330

Purpose

Triggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-HA4-7c12 Tandem Monobody

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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IGSF5_pCSdest

Plasmid

#53886

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

IGSF5 (IGSF5 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
JN607: pMVP/SB-DEST

Plasmid

#121860

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KK402: pMVP/Zeo-DEST

Plasmid

#121859

Purpose

pMVP destination vector, empty expression vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Myt1

Plasmid

#64845

Purpose

A lentiviral vector for the expression of mouse Myt1 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Myt1 (Myt1 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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CYYR1_pCSdest

Plasmid

#53794

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CYYR1 (CYYR1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
KP301: pMVP (L5-L4) mCherry-P2A

Plasmid

#121710

Purpose

pMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry-P2A (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pFAB803

Plasmid

#47847

Purpose

BIOFAB reporter plasmid for measuring T7 early termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

T7 early terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO2

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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