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pLLU2G-Oct3

Plasmid

#21616

Depositor

David Garbers

Publication

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Oct3 shRNA

Use

Lentiviral and RNAi

Available since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pLLNeo

Plasmid

#21617

Depositor

David Garbers

Publication

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Type

Empty backbone

Use

Lentiviral and RNAi

Available since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-polyQ74-NLS

Plasmid

#231892

Purpose

Forms nuclear HTT partial exon 1 Q74-NLS aggregates

Depositor

Lukas Kapitein

Publication

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74-NLS (HTT Human)

Tags

EGFP and SV40 NLS

Expression

Mammalian

Mutation

CAG repeat expansion

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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Mutant Human Cortactin-GFP

Plasmid

#71365

Purpose

mammalian expression of mutant human Cortactin-GFP

Depositor

Alpha Yap

Publication

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

cortactin (CTTN Human)

Tags

EGFP

Expression

Mammalian

Mutation

silent mutation present at 72nd nucleotide

Promoter

CMV

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[1] (mammalian)

Plasmid

#52901

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Publication

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[2] (mammalian)

Plasmid

#52902

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Publication

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-PPO-mScarlet

Plasmid

#139503

Purpose

Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Publication

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Tags

mScarlet following Gly-Gly-Ser linker

Expression

Mammalian

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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M4757 KanMX::TRP1 Disruptor Converter

Plasmid

#51687

Purpose

Yeast marker swap plasmid for converting KanMX3 to TRP1

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

TRP1 (TRP1 Budding Yeast)

Expression

Yeast

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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M2660 ura3::LYS2 Disruptor Converter

Plasmid

#51678

Purpose

Yeast marker swap plasmid for converting URA3 to LYS2

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LYS2 (LYS2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA GAL4 CREB 1-341 M1

Plasmid

#22973

Depositor

Marc Montminy

Publication

Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

Insert

CREB (Creb1 GAL4-fusion, Rat)

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine of CREB

Available since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MEIS1B-pSP65

Plasmid

#21019

Depositor

Corey Largman

Publication

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1B (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-4T MDM2 S186D

Plasmid

#16239

Depositor

Mien-Chie Hung

Publication

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Tags

GST

Expression

Bacterial

Mutation

Ser186 replaced by Asp

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
HOXD12 (murine) pRSETa

Plasmid

#8554

Depositor

Corey Largman

Publication

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

HOXD12 (Hoxd12 Mouse)

Tags

HIS and XPress

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
TDP-43 WT GFP

Plasmid

#237542

Purpose

Expression of GFP-tag fused to wild-type TDP-43

Depositor

Tatyana Shelkovnikova

Publication

Matsukawa et al RNA Biol. 2021 Jan 11:1-9. doi: 10.1080/

Insert

GFP-tagged TDP-43 WT (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

wild-type TDP-43

Promoter

CMV

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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negActinin-sstFRET-GR

Plasmid

#83417

Purpose

FRET-based actinin tension sensor (negative control)

Depositor

Tetsuya Kitaguchi

Publication

Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

Insert

negActinin-sstFRET

Expression

Mammalian

Promoter

CMV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
pReporter_8

Plasmid

#60568

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_8

Expression

Bacterial

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAS166

Plasmid

#194489

Purpose

43-8 Op (Part 2)

Depositor

Ahmad Khalil

Publication

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

43-8 Op (Part 2)

Use

Synthetic Biology

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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SCP2-pUC119

Plasmid

#21951

Depositor

James Kadonaga

Publication

Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

Insert

SCP2 (super core promoter 2)-pUC119

Use

A puc derivative - a cloning vector

Available since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pRB2070

Plasmid

#8671

Depositor

David Botstein

Publication

Richards et al Mol Biol Cell 2000 May;11(5):1887-903.

Insert

TUB1 with LYS2 (TUB1 Budding Yeast)

Expression

Yeast

Available since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
MAC-DPP4

Plasmid

#172416

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Dipeptidyl peptidase 4 (DPP4 Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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