We narrowed to 11,279 results for: Vars

1. OST +Q111R

   Plasmid

   #187436

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   changed glutamine at 116 to arginine

   Promoter

   PH and p10

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. FER +H111T

   Plasmid

   #187430

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   changed histidine at 117 to threonine

   Promoter

   PH and p10

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CHI +N122D

   Plasmid

   #187427

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   changed asparagine at 130 to aspartic acid

   Promoter

   PH and p10

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. NODAL-MYC-DYK-N72A-N199A

   Plasmid

   #115252

   Purpose

   For mammalian expression of the human NODAL open reading frame (with two mutated N-glycosylation sites) with a C-terminal MYC-DYK tag

   Depositor

   Lynne Postovit

   Article

   Findlay et al (2018) bioRxiv 276170

   Insert

   NODAL (NODAL Human)
   

   Use

   Tags

   MYC-DYK

   Expression

   Mammalian

   Mutation

   N72A, N199A

   Promoter

   CMV

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. NODAL-MYC-DYK-C312A

   Plasmid

   #115253

   Purpose

   For mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag

   Depositor

   Lynne Postovit

   Article

   Findlay et al (2018) bioRxiv 276170

   Insert

   NODAL (NODAL Human)
   

   Use

   Tags

   MYC-DYK

   Expression

   Mammalian

   Mutation

   C312A

   Promoter

   CMV

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisplay-V5-Nxph1

   Plasmid

   #184336

   Purpose

   Expression of V5-tagged mouse neurexophilin-1

   Depositor

   Peter Scheiffele

   Article

   Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

   Insert

   neurexophilin-1 (Nxph1 Mouse)
   

   Use

   Tags

   V5-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GST_LIM1224

   Plasmid

   #182041

   Purpose

   For expression of human paxillin LIM domains 1, 2, 2 and 4 with N-terminal GST fusion protein in E. coli.

   Depositor

   Vesa Hytönen

   Article

   Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

   Insert

   LIM1224 (PXN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   LIM domains 1, 2, 2 and 4

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. GST_LIM1334

   Plasmid

   #182040

   Purpose

   For expression of human paxillin LIM domains 1, 3, 3 and 4 with N-terminal GST fusion protein in E. coli.

   Depositor

   Vesa Hytönen

   Article

   Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

   Insert

   LIM1334 (PXN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   LIM domains 1, 3, 3 and 4

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pML104-natR412

   Plasmid

   #162042

   Purpose

   Expresses Cas9 and contains natR412 guide which targets the natR gene

   Depositor

   Jolanda Van Leeuwen

   Article

   Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

   Insert

   natR412 guide
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pML107-natR854

    Plasmid

    #162043

    Purpose

    Expresses Cas9 and contains natR854 guide which targets the natR gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

    Insert

    natR854 guide
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lmajor_gp63_10_0460_P460G_D463N_S465A

    Plasmid

    #171645

    Purpose

    Expression of L. major glcyoprotein-63 (P460G_D463N_S465A) with substrate binding site mutations from chromosome 10 in mammalian cells

    Depositor

    Dennis Ko

    Article

    Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

    Insert

    LmjF.10.0460 P460G D463N S465A
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    P460G; D463N; S465A

    Promoter

    Available since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJH191

    Plasmid

    #172149

    Purpose

    Accessory Plasmid for PACE of pylBCD variants

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Ppsp gIII.2TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    gIII(P29*, Y184*); [strong promoter eG, strong RB…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJH190

    Plasmid

    #172148

    Purpose

    Accessory Plasmid for PACE of pylBCD variants

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    gIII(P29*); [strong promoter eG, strong RBS SD8] …

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Negative feedback (NF) plasmid

    Plasmid

    #169747

    Purpose

    Standalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tetO2

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMS11: pTns(AvCAST)_ΔTniQ,ΔTnsD

    Plasmid

    #168144

    Purpose

    Inducible expression of AvCAST TnsA, TnsB and TnsC proteins.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST Tns proteins (TnsA, TnsB and TnsC)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMS9: pTns(AvCAST)_ΔTniQ

    Plasmid

    #168142

    Purpose

    Inducible expression of AvCAST TnsA, TnsB, TnsC and TnsD proteins.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST Tns proteins (TnsA, TnsB and TnsC)
    AvTnsD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCRISPR V2 sgACO1_3

    Plasmid

    #169893

    Purpose

    Disrupt ACO1

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    sgRNA targeting ACO1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgiRFP1

    Plasmid

    #113972

    Purpose

    Single short guide RNA targeting GATCGAGTTCGAGCCTGCGG in iRFP670 sequence

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    iRFP670 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgiRFP2

    Plasmid

    #113973

    Purpose

    Single short guide RNA targeting GCGCGTTCTTTGGACGCGA in iRFP670 sequence

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    iRFP670 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgiRFP3

    Plasmid

    #113974

    Purpose

    Single short guide RNA targeting CGTGATGTTGTACCGCTTC in iRFP670 sequence

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    iRFP670 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge