We narrowed to 3,553 results for: lenti crispr cas9 plasmids

1. pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

   Plasmid

   #83925

   Purpose

   Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   eGFP
   Bsr
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A

   Expression

   Mutation

   Promoter

   hUbC

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO5.sgRNA.EFS.GFP

   Plasmid

   #57822

   Purpose

   3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLKO5.sgRNA.EFS.tRFP

   Plasmid

   #57823

   Purpose

   3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   tagRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pXPR_502

   Plasmid

   #96923

   Purpose

   for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF

   Depositor

   David Root, John Doench

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pXPR_501

   Plasmid

   #96922

   Purpose

   for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64

   Depositor

   David Root, John Doench

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPapi

   Plasmid

   #96921

   Purpose

   expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.

   Depositor

   David Root, John Doench

   Article

   Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

   Plasmid

   #83919

   Purpose

   Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   DsRed-Express2
   Bsr
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A

   Expression

   Mutation

   Promoter

   hUbC

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO5.sgRNA.EFS.PAC

   Plasmid

   #57825

   Purpose

   Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJRH051

   Plasmid

   #171625

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BsmBI-Destination
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSECC

    Plasmid

    #60820

    Purpose

    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

    Depositor

    Tyler Jacks

    Article

    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

    Inserts

    Cas9
    Cre
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    BsmBI site eliminated by C->A; E308G

    Promoter

    EFS and EFS (after Cas9-2A)

    Available since

    Nov. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAT005

    Plasmid

    #171632

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-B2M
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJRH623

    Plasmid

    #171633

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-non-targeting
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAT003

    Plasmid

    #171628

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-TRAC
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1-a

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. SGL40C.EFS.E2Crimson

    Plasmid

    #100894

    Purpose

    Lentiviral gRNA delivery

    Depositor

    Dirk Heckl

    Article

    Labuhn et al Nucleic Acids Res. 2018 Feb 16;46(3):137

    Insert

    E2Crimson
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. EXOSC10 gRNA (BRDN0001148841)

    Plasmid

    #77989

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EXOSC10

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EXOSC10 (EXOSC10 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EXOSC10 gRNA (BRDN0001149316)

    Plasmid

    #77990

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EXOSC10

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EXOSC10 (EXOSC10 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EXOSC10 gRNA (BRDN0001487044)

    Plasmid

    #77988

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EXOSC10

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EXOSC10 (EXOSC10 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EXOSC10 gRNA (BRDN0001147008)

    Plasmid

    #77987

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EXOSC10

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EXOSC10 (EXOSC10 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ERBB2 gRNA (BRDN0001146057)

    Plasmid

    #76376

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ERBB2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MAGI1 gRNA (BRDN0001146833)

    Plasmid

    #78067

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAGI1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge