Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 5,100 results for: luciferase

Showing: 3321 - 3340 of 5100 results

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psiCHECK-1-miR-376a1(5p)-I-type

Plasmid

#215882

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of 376a1(5p)-I-type (MIR376A1 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a1(3p)-I-type

Plasmid

#215883

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-I-type (MIR376A1 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-589(5p)-I-type

Plasmid

#215884

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-589(5p)-I-type (MIR589 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-589(3p)-I-type

Plasmid

#215885

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-589(3p)-I-type (MIR589 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-22(3p)-I-type

Plasmid

#215886

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-22(3p)-I-type (MIR22 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a2(3p)-I-type

Plasmid

#215887

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-I-type (MIR376A2 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-99a-I-type

Plasmid

#215888

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-99a-I-type (MIR99A Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-PILATeS-RGD (200nM)

Plasmid

#216716

Purpose

Expresses HaloTag-PILATeS-RGD with 200 nM BiT tag in bacteria

Depositor

Alex Dunn

Publication

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

HaloTag-PILATeS-RGD

Tags

6xHis and HaloTag

Expression

Bacterial

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-PILATeS-RGD (700pM)

Plasmid

#216714

Purpose

Expresses HaloTag-PILATeS-RGD with 700 pM BiT tag in bacteria

Depositor

Alex Dunn

Publication

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

HaloTag-PILATeS-RGD

Tags

6xHis and HaloTag

Expression

Bacterial

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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FB398

Plasmid

#203634

Purpose

Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).

Depositor

Jose Marcos

Publication

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

FB367+FB395

Use

Synthetic Biology

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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NK810

Plasmid

#196407

Purpose

Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control

Depositor

Nicole King

Publication

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin

Use

Luciferase

Mutation

N/A (synthetic)

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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psiCheck Cdk2ap1ΔN (MT2B2)

Plasmid

#178029

Purpose

expression vector to test translation efficiency by luciferase reporter.

Depositor

Lin He

Publication

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Luciferase; Nonviral

Tags

Luciferase

Expression

Mammalian

Promoter

Cloned in

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-ZC3H12D_PromoterMUT

Plasmid

#153067

Purpose

ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter

Depositor

Silvia Monticelli

Publication

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutagenesis to disrupt 3 BHLHE4040 binding sites.…

Promoter

None

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR-IL22

Plasmid

#153070

Purpose

Human IL-22 3'UTR region cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Publication

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

3' UTR region of IL-22 gene (IL22 Human)

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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PAK4-3'UTR-BS2

Plasmid

#113091

Purpose

Luciferase reporter containing PAK4 3' UTR with the second predicted MiR-199a-3p binding

Depositor

James Donahue

Publication

Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

Insert

p21 Activated kinase 4 (PAK4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

none

Promoter

PGK

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Fascin-Promoter (-210-0)

Plasmid

#89826

Purpose

Fascin promoter for luciferase assay

Depositor

Shengyu Yang

Publication

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Promoter

SV40

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P6

Plasmid

#64790

Purpose

Luciferase expression driven by miR-34a promoter P6

Depositor

Joshua Mendell

Publication

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P6 (MIR34A Human)

Use

Luciferase

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P3

Plasmid

#64787

Purpose

Luciferase expression driven by miR-34a promoter P3

Depositor

Joshua Mendell

Publication

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P3 (MIR34A Human)

Use

Luciferase

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P5

Plasmid

#64789

Purpose

Luciferase expression driven by miR-34a promoter P5

Depositor

Joshua Mendell

Publication

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P5 (MIR34A Human)

Use

Luciferase

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P4

Plasmid

#64788

Purpose

Luciferase expression driven by miR-34a promoter P4

Depositor

Joshua Mendell

Publication

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P4 (MIR34A Human)

Use

Luciferase

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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Showing: 3321 - 3340 of 5100 results

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