We narrowed to 6,073 results for: Drosophila

Showing: 3341 - 3360 of 6073 results

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pET28a_DmKHC[1-421]-1xGFP-6xHis

Plasmid

#196972

Purpose

Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

Depositor

Lukas Kapitein

Publication

Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

Insert

Kinesin Heavy Chain (Khc Fly)

Tags

6xHis and GFP

Expression

Bacterial

Promoter

T7 promoter

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Mtl DN

Plasmid

#52252

Purpose

Drosophila expression of YFP-Mtl dominant negative mutant

Depositor

Matthew Scott

Insert

Mtl (Mtl Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T20N; DN mutation

Promoter

GAL4

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
pUASp YFP Mtl CA

Plasmid

#52253

Purpose

Drosophila expression of YFP-Mtl constitutively active mutant

Depositor

Matthew Scott

Insert

Mtl (Mtl Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q64L; CA mutation

Promoter

GAL4

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rac2 CA

Plasmid

#52238

Purpose

Drosophila expression of YFP-Rac2 constitutively active mutant

Depositor

Matthew Scott

Insert

Rac2 (Rac2 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q61L; CA mutation

Promoter

GAL4

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rac2 DN

Plasmid

#52237

Purpose

Drosophila expression of YFP-Rac2 dominant negative mutant

Depositor

Matthew Scott

Insert

Rac2 (Rac2 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T17N; DN mutation

Promoter

GAL4

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
OA-986B

Plasmid

#124999

Purpose

Expresses dCas9-VPR driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Publication

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Expression

Insect

Promoter

Ubiquitin

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b Me31B-3Myc

Plasmid

#113001

Purpose

For protein expression and purification of Myc-tagged full-length Drosophila me31B

Depositor

Terry Orr-Weaver

Publication

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length me31B (me31B Fly)

Tags

Myc

Expression

Bacterial

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pKM19

Plasmid

#123655

Purpose

Encodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cells

Depositor

Kevin Maringer

Publication

Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

Insert

Firefly luciferase

Use

Luciferase

Expression

Insect

Promoter

pIEx

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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AAEL003877-Cas9

Plasmid

#100581

Purpose

The promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Publication

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL003877-hspcas9-GFP
Opie-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL003877 and Opie2

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CS2-GFP-nopo

Plasmid

#47964

Purpose

Expression of Fly nopo

Depositor

Laura Lee

Publication

Merkle et al Development. 2009 Feb;136(3):449-59. doi

Insert

no poles (nopo Fly)

Tags

GFP

Expression

Mammalian

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
hs-FL-Kek1-myc2

Plasmid

#41170

Depositor

Norbert Perrimon

Publication

Ghiglione et al Cell. 1999 Mar 19;96(6):847-56.

Insert

Kek1 (kek1 Fly)

Tags

2X myc

Expression

Insect

Promoter

Hsp70

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
Torso 11.5 kb rescue fragment

Plasmid

#39810

Depositor

Norbert Perrimon

Publication

Gayko et al Proc Natl Acad Sci U S A. 1999 Jan 19;96

Insert

Torso genomic DNA fragment (tor Fly)

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
Torso cDNA Y918F in pGEM

Plasmid

#39797

Depositor

Norbert Perrimon

Publication

Gayko et al Proc Natl Acad Sci U S A. 1999 Jan 19;96

Insert

Torso (tor Fly)

Expression

Bacterial

Mutation

Y918F

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pRmHa3-dKDM4A-H195A-HA2FL2

Plasmid

#20235

Depositor

Jerry Workman

Publication

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG2 and HA2

Expression

Insect

Mutation

changed His 195 to Ala

Available since

March 19, 2009

Availability

Academic Institutions and Nonprofits only
pBacPAK-HFT-dKDM4A-H195A

Plasmid

#20236

Depositor

Jerry Workman

Publication

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG, His, and TEV

Expression

Insect

Mutation

changed His 195 to Ala

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pET28a-MED11

Plasmid

#15414

Depositor

Joan Conaway, Ronald Conaway

Publication

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED11 (Med11 Mouse)

Tags

His

Expression

Bacterial

Mutation

N/A

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pUAST myc Rab35 CA

Plasmid

#58382

Purpose

expression of drosophila myc-Rab35 constitutively active mutant

Depositor

Matthew Scott

Publication

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Fly)

Tags

myc

Expression

Insect

Mutation

Q67L (constitutive active)

Promoter

GAL4

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pUAST YFP Rab35 CA

Plasmid

#58381

Purpose

expression of drosophila YFP-Rab35 constitutively active mutant

Depositor

Matthew Scott

Publication

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Fly)

Tags

YFP

Expression

Insect

Mutation

Q67L (constitutive active)

Promoter

GAL4

Available since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pDSARN-vasa-eSpCas9sv40

Plasmid

#173670

Purpose

eSpCas9 expression vector for Anopheles

Depositor

Eric Marois

Publication

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Insert

eSpCas9 with Anopheles vasa promoter and SV40 terminator

Use

CRISPR

Expression

Insect

Mutation

mutations in Cas9 reducing off-target activity (S…

Promoter

vasa

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159673

Purpose

Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Publication

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Tags

T2A-GFP

Expression

Insect

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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