We narrowed to 9,000 results for: ef1;

1. Aiolos ZF2 (AA146-168) in Cilantro 2

   Plasmid

   #74456

   Purpose

   Monitor post-translational degradation of Aiolos ZF2 via EGFP:mCherry ratio (Lentiviral, PGK.AiolosAA146-168-10AAFlexLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

   Depositor

   Benjamin Ebert

   Insert

   Aiolos (IKZF3) AA130-189 (IKZF3 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG/HA-hBIG1

   Plasmid

   #226253

   Purpose

   Expresses N-terminally HA-tagged hBIG1 in mammalian cells

   Depositor

   Hye-Won Shin

   Insert

   BIG1 (ARFGEF1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-Myc-TEAD1-Y406A

   Plasmid

   #33047

   Depositor

   Kunliang Guan

   Article

   Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

   Insert

   TEAD1 (TEAD1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Y406A

   Promoter

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pKAM-E-Flag-BFP

   Plasmid

   #101867

   Purpose

   Lentiviral Evi-1-Flag with dual BFP and puro markers

   Depositor

   Archibald Perkins

   Insert

   Evi-1 (Mecom Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPEE8

   Plasmid

   #128358

   Purpose

   Recycable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by direct repeats.

   Depositor

   James Fraser

   Article

   Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   TEF1

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKAM-E-Flag-GFP

   Plasmid

   #101866

   Purpose

   Lentiviral Evi-1-Flag with GFP marker

   Depositor

   Archibald Perkins

   Insert

   Evi-1 (Mecom Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPL5617_pUG_PuroR

   Plasmid

   #60928

   Purpose

   PCR template vector for generating puromycin resistance cassette.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   puromycin N-Acetyltransferase
   

   Use

   Loxp flanked deletion cassette template

   Tags

   Expression

   Mutation

   Promoter

   TEF1

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. SOX17-tdTomato-EhB

   Plasmid

   #210467

   Purpose

   Donor plasmid for knock-in tdTomato into the human SOX17 locus

   Depositor

   Kai Wang

   Insert

   SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived hygromycin-NLS-mTagBFP2 selection cassette (SOX17 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGBW-m4046708

   Plasmid

   #149173

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pTEAD1.1.0-gDNA

    Plasmid

    #112425

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TEAD1

    Depositor

    Kevin White

    Insert

    TEAD1 (TEAD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEF-GalNAcT2-moxGFP2

    Plasmid

    #218966

    Purpose

    Expresses full length GalNAcT2-moxGFP2 mammalian cells

    Depositor

    Benjamin Glick

    Insert

    GalNAcT2-moxGFP2 (GALNT2 Human)
    

    Use

    Tags

    moxGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV Cbx1-V5

    Plasmid

    #175106

    Purpose

    Lentiviral expression of mouse Cbx1-V5

    Depositor

    Larry Gerace

    Insert

    Cbx1 (Cbx1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEFX-AP

    Plasmid

    #31234

    Depositor

    Cliff Ragsdale

    Article

    Agarwala et al Science. 2001 Mar 16. 291(5511):2147-50.

    Insert

    AP (ALPP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pJA317

    Plasmid

    #74488

    Purpose

    eF1alpha::puroR::mCherry::T2A::eGFP::d1ODC::wPRE has d1ODC degron

    Depositor

    Andrew Fire

    Article

    Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

    Insert

    d1ODC
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKL08

    Plasmid

    #104432

    Purpose

    pHuji in yeast expression vector

    Depositor

    Joel Kralj

    Article

    Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

    Insert

    pHuji
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEBB-TB

    Plasmid

    #36099

    Depositor

    Ezra Burstein

    Article

    Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

    Type

    Empty backbone

    Use

    Tags

    BT-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJA318

    Plasmid

    #74489

    Purpose

    eF1alpha::puroR::mCherry::T2A::eGFP::d4ODC::wPRE has d4ODC degron

    Depositor

    Andrew Fire

    Article

    Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

    Insert

    d4ODC
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGBW-m4046327

    Plasmid

    #153913

    Purpose

    Yeast expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046632

    Plasmid

    #153929

    Purpose

    Yeast expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046300

    Plasmid

    #149043

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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