We narrowed to 7,394 results for: 11

1. pSIN-Skil-gRNA2

   Plasmid

   #180371

   Purpose

   targeting mouse Skil/SnoN gene

   Depositor

   Dan Littman

   Article

   Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

   Insert

   Skil targeting gRNA (Skil Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CreLite

   Plasmid

   #131786

   Purpose

   Lentiviral vector expressing CreLite, driven by CBh promoter

   Depositor

   George Eisenhoffer

   Article

   Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

   Insert

   PhyBCreC-P2A-PIF6CreN
   

   Use

   Cre/Lox, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK234_032

   Plasmid

   #123995

   Purpose

   Encodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA1 -hAAVS1
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. hFLT1 delta CTD

   Plasmid

   #86036

   Purpose

   human VEGFR1 with deletion of the entire intracellular domain

   Depositor

   Christie Thomas

   Article

   Raikwar et al PLoS One. 2014 Nov 11;9(11):e112794. doi

   Insert

   human Flt1 delta CTD (FLT1 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   deta CTD

   Promoter

   CMV

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. evoCas9

   Plasmid

   #138558

   Purpose

   Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   evoCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK234_033

   Plasmid

   #123996

   Purpose

   Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA2 -hAAVS1
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK234_034

   Plasmid

   #123997

   Purpose

   Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA3 -hAAVS1
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MTK0_031

   Plasmid

   #123970

   Purpose

   Encodes the BxBI-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   BxBI-hygro-mRuby2PEST landing
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSIN-Ski-gRNA2

   Plasmid

   #180369

   Purpose

   targeting mouse Ski gene

   Depositor

   Dan Littman

   Article

   Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

   Insert

   Ski targeting gRNA (Ski Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Keasling-2187

    Plasmid

    #100167

    Purpose

    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

    Depositor

    Jay Keasling

    Article

    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

    Insert

    MevT and MBIS operon
    

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. TALEN-CCR5L18-CtermQ7

    Plasmid

    #51442

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with Q7 C-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) Q7 C-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Mutation

    K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. ATF4 7: 5’3’ATF4.GFP

    Plasmid

    #21854

    Depositor

    David Ron

    Article

    Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

    Insert

    ATF4 (Atf4 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Vector derived viral termination and poly-adenyla…

    Available Since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-HA-REDD1 48-232 (#182)

    Plasmid

    #99920

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal deletion; encodes AA 48-232

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-GP-2-MPHOSPH8 Chromo

    Plasmid

    #59694

    Purpose

    Contains histone modification interacting domain (MPHOSPH8 Chromo)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    MPHOSPH8 Chromo (MPHOSPH8 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPuroR-MCS-LexA-VP16-MiniWhite

    Plasmid

    #165898

    Purpose

    Puromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbB8k-csg-NbMS2

    Plasmid

    #171701

    Purpose

    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.

    Depositor

    Neel Joshi

    Article

    Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1

    Insert

    csgBACEFG, with nanobody fused to CsgA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PML-ER-pBabe

    Plasmid

    #73556

    Purpose

    vector encoding a fusion protein between PML-IV and ER (estrogen receptor)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    PML-IV (PML Human)
    

    Use

    Retroviral

    Tags

    ER

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI

    Plasmid

    #140202

    Purpose

    CRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology; Proof-of-concept te…

    Mutation

    G174D

    Promoter

    PelcA

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. VQR variant

    Plasmid

    #138560

    Purpose

    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VQR
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEasyG3_nat

    Plasmid

    #184918

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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