We narrowed to 4,471 results for: ARA-2
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Plasmid#127793PurposeExpresses the CHAD domain of ygiF in E. coli cellsDepositorInsertygiF-CHAD (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 201-433)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1527 [IBB-GFP-mCherry3E]-[BFP-TDP43 W-S]
Plasmid#133333PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll W to S in CTD of full-length TDP43PromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1410 GFP-TDP43 F-S
Plasmid#118799PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralTagsExpressionMutationAll F to S in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1396 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT]
Plasmid#118804PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1388 TDP43 RRM-GFP K-R
Plasmid#118796PurposeTo test the effect of sequence on TDP43 RRM-GFP droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll K to R in CTD of TDP43 RRM-GFP reporterPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opie2-NeoR[traF]+Opie2-PuroR[dsxM]
Plasmid#131618PurposeExpresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.DepositorInsertsPuroR[dsxM]
NeoR[traF]
UseTagsExpressionInsectMutationPromoterOpie2Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHBS931 TDP43 RRM-GFP F-S
Plasmid#107799PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll phenylalanine to serinePromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-NOT3 isoform A (HK100)
Plasmid#206457PurposeNOT3 expression in Schneider cells for imagingDepositorInsertNOT3 (Not3 Fly)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-mEGFP-NOT3 isoform A (HK55)
Plasmid#206442PurposeNOT3 expression in Schneider cells for imagingDepositorInsertNOT3 (Not3 Fly)
UseTagsmEGFPExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mEGFP-Cup isoform B (JM051)
Plasmid#206451PurposeCup expression in Schneider cells for imagingDepositorInsertCup (cup Fly)
UseTagsPH mEGFPExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUiLMO2W
Plasmid#129480Purpose3rd-generation lentiviral vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemicalDepositorInsertiLMO2
UseLentiviralTagsExpressionMammalianMutationPromoterhuman ubiquitin CAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ZmIAA29 (yeast codon optimized)
Plasmid#188441PurposeZea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimizationDepositorInsertAuxin Signaling Repressor IAA29 (yeast codon optimized)
UseCloningTagsExpressionMutationPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_SsCHAD-H29A-R32A-R36A
Plasmid#127786PurposeExpresses a SsCHAD mutant protein in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationmutations in His29, Arg32 and Arg36 to AlaPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-R438A-K441A
Plasmid#127791PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in Arg4…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-TTM
Plasmid#127794PurposeExpresses the TTM/CYTH domain of ygiF in E. coli cellsDepositorInsertygiF (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 1-200)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS934 TDP43 RRM-GFP M-V
Plasmid#107801PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll methionine to valine in LLPS reporterPromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS935 TDP43 RRM-GFP M-S
Plasmid#107800PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll methionine to serine in LLPS reporterPromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1052 TDP43 RRM-GFP R-K
Plasmid#107856PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll arginine to lysine in LLPS reporterPromoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
lucMAPT-GenRep
Plasmid#214674PurposeMAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exonsDepositorInsertMAPT (MAPT Human)
UseTagsFirefly Luciferase, Renilla Luciferase, and V5ExpressionMammalianMutationContains Exon 9 and Exon 11, with a BamHI EcoRI c…PromoterCMVAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)
Plasmid#187844PurposeExpression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
UseTagsIg k-chain leader sequence, SNAPf-tag, and meGFPe…ExpressionMammalianMutationmeGFPe: asparagine 198 to aspartic acid and tyros…PromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only